Product Name	Caspase-12
Chinese Name	半胱胺酸蛋白酶蛋白-12抗体
Alias	UNQ9415; Apoptosis related cysteine protease; CASP 12; casp12; CASP12P1; Caspase 12 pseudogene 1; CASP-12; casp12; caspase 12 (gene/pseudogene); Caspase12; CASPC_HUMAN; OTTHUMP00000207026; OTTHUMP00000207028; OTTHUMP00000207031; OTTHUMP00000207032; OTTHUMP00000219058; UNQ9415.
literatures	Specific References  (23)     |     SL1105R has been referenced in 23 publications. [IF=7.129] Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186  WB ;  Rat, Human.   PubMed:36244175 [IF=6.706] Yujie Zhong. et al. Diosgenin Ameliorated Type II Diabetes-Associated Nonalcoholic Fatty Liver Disease through Inhibiting De Novo Lipogenesis and Improving Fatty Acid Oxidation and Mitochondrial Function in Rats. NUTRIENTS. 2022 Jan;14(23):4994  IHC ;  Rat.   PubMed:36501024 [IF=6.706] Yujie Zhong. et al. Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy. NUTRIENTS. 2023 Jan;15(9):2164  WB ;  Rat,Human.   PubMed:10.3390/nu15092164 [IF=6.284] Yujie Zhong. et al. Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca 2+ homeostasis. 2021 Mar 02  WB ;  Rat.   PubMed:33651226 [IF=6.175] Chen Yanan. et al. Pterostilbene attenuates intrauterine growth retardation-induced colon inflammation in piglets by modulating endoplasmic reticulum stress and autophagy. Journal of Animal Science and Biotechnology. 2022 Dec;13(1):1-16  WB ;  Human.   PubMed:36329539 [IF=6.117] Huawu Gao. et al. Genistein attenuates memory impairment in Alzheimer's disease via ERS-mediated apoptotic pathway in vivo and in vitro. J NUTR BIOCHEM. 2022 Aug;:109118  WB ;  Rat.   PubMed:35933022 [IF=6.023] Yujie Zhong. et al. Jujuboside A ameliorates high fat diet and streptozotocin induced diabetic nephropathy via suppressing oxidative stress, apoptosis, and enhancing autophagy. Food Chem Toxicol. 2022 Jan;159:112697  WB ;  Rat.   PubMed:34826549 [IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551  WB ;  Mouse.   PubMed:32996162 [IF=5.396] Yujie Zhong. et al. Dioscin relieves diabetic nephropathy via suppressing oxidative stress, apoptosis, and improving mitochondrial quality and quantity control. Food Funct. 2022 Feb;:  WB ;  Rat.   PubMed:35262539 [IF=4.784] Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26.  IHC-P ;  Mouse.   PubMed:30605745 [IF=4.65] Yu, H., et al. "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro."Cellular Physiology and Biochemistry 39.1 (2016): 123-136.  WB ;  Rat.   PubMed:27322831 [IF=4.096] Xiaowei Sun. et al. Acupuncture protects against cerebral ischemia–reperfusion injury via suppressing endoplasmic reticulum stress-mediated autophagy and apoptosis. Mol Med. 2020 Dec;26(1):1-14  WB ;  Rat.   PubMed:33167857 [IF=3.448] Sun Y et al. Effect of endoplasmic reticulum stress-mediated excessive autophagy on apoptosis and formation of kidney stones. Life Sci. 2019 Dec 26:117232.  WB ;  Rat.   PubMed:31884097 [IF=3.23] Wang, Yu, et al. "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." PloS one 12.4 (2017): e0173469.  WB ;  Rat.   PubMed:28399139 [IF=3.219] Yuting Li. et al. Evodiamine suppresses the progression of non-small cell lung carcinoma via endoplasmic reticulum stress-mediated apoptosis pathway in vivo and in vitro:. INT J IMMUNOPATH PH. Int J Immunopath Ph. 2022;():  WB ;  Human,Mouse.   PubMed:35388733 [IF=2.29] Wang, Yu, et al. "Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways." Heart and Vessels (2016): 1-8.  WB ;  Rat.   PubMed:27639990 [IF=2.2] Zhang, Huiying, et al. "Glucose-regulated protein 78 may play a crucial role in promoting the pulmonary microvascular remodeling in a rat model of hepatopulmonary syndrome." Gene (2014).  IHC-P ;  Rat.   PubMed:24768185 [IF=2.197] Luo Q et al. Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in PolyphyllinI-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells. Biomed Res Int. 2018 Dec 25;2018:5241941.  WB ;  Human.   PubMed:30671458 [IF=1.89] Zhang, Lili, et al. "Increased expression of 78 kD glucose-regulated protein promotes cardiomyocyte apoptosis in a rat model of liver cirrhosis." International Journal of Clinical and Experimental Pathology 8.8 (2015): 9256-9263.  IHC-P ;  Rat.   PubMed:26464674 [IF=1.88] Zhou, Yunchuan, et al. "Sulfiredoxin-1 exerts anti-apoptotic and neuroprotective effects against oxidative stress-induced injury in rat cortical astrocytes following exposure to oxygen-glucose deprivation and hydrogen peroxide."International Journal of Molecular Medicine (2015).  WB ;  Rat.   PubMed:25955519 [IF=1.798] Wei-Ting Xuanet al. Berberine ameliorates rats model of combined Alzheimer’s disease and type 2 diabetes mellitus via the suppression of endoplasmic reticulum stress. 3 Biotech . 2020 Aug;10(8):359.  WB ;  rat.   PubMed:32832321 [IF=1.665] Rui Zhang. et al. Apigetrin ameliorates streptozotocin-induced pancreatic β-cell damages via attenuating endoplasmic reticulum stress. In Vitro Cell Dev-An. 2020 Sep;56(8):622-634  WB ;  Rat.   PubMed:32901429 [IF=0.504] Zhongkui Li. et al. Down-modulation of endoplasmic reticulum stressinitiated apoptosis by huperzine A in isoproterenolprovoked myocardial infarction rat model: Role of Nrf2/HO- 1 signaling axis. Trop J Pharm Res. 2020 Nov;19(8):1685-1691  IHC ;  Rat.   PubMed:10.4314/tjpr.v19i8.17
Research Area	Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	46kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from the middle of human Caspase-12: 201-300/420
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]. Function: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Tissue Specificity: Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: O08736 Gene ID: 100506742 Database links: Entrez Gene: 100506742 Human Entrez Gene: 12364 Mouse Entrez Gene: 156117 Rat SwissProt: Q6UXS9 Human SwissProt: O08736 Mouse SwissProt: Q920D5 Rat Omim: 608633 Human Unigene: 476989 Human Unigene: 81078 Rat Caspase-12是caspase家族成员，是存在于内质网上的促凋亡因子，内质网应激早期可通过GRP（glucose-regulated protein，GRP）的表达增多来保护细胞；CASPASE-12是内质网细胞关键介导因子，caspase-12还是caspase-1的抑制剂。
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-alpha smooth muscle Actin (SL1105R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Caspase-12 (SL1105R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD Sample: Lane 1: Muscle (Mouse) Tissue Lysate at 40 ug Lane 2: Muscle (Rat) Tissue Lysate at 40 ug Primary: Anti-Caspase-12 (SL1105R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 47/40 kD Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Kidney (Mouse) Lysate at 40 ug Primary: Anti-Caspase-12 (SL1105R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45/52 kD Observed band size: 46 kD Paraformaldehyde-fixed, paraffin embedded (mouse fallopian tube); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (SL1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase-12) Polyclonal Antibody, Unconjugated (SL1105R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Caspase-12 Polyclonal Antibody, Unconjugated(SL1105R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat testis tissue(high fat); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(SLR) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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