Product Name	Gliomedin
Chinese Name	神经胶质蛋白(肝癌相关基因2)抗体
Alias	CANCER RELATED GENE LIVER 2; Cancer related gene-Liver 2; CLOM; collomin; Colm (gene name); COLM; Colmedin; CRG L2; FLJ23917; Gldn (gene name); UNC 112; GLDN_HUMAN.
Research Area	Neurobiology  Signal transduction  Cell adhesion molecule  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	59kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Gliomedin/COLM: 365-460/551 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Gliomedin is a 551 amino acid protein encoded by the human gene GLDN. Gliomedin is thought to play a role in the formation of the nodes of Ranvier along myelinated axons. Accumulation of Na+ channels at the nodes of Ranvier is a prerequisite for saltatory conduction. In peripheral nerves, clustering of these channels along the axolemma is regulated by myelinating Schwann cells through an unknown mechanism. Gliomedin is a glial ligand for Neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes. Gliomedin is a single-pass type II membrane protein localized to the nodes of Ranvier and is specifically expressed in spinal cord, brain, placenta and sciatic nerve. It is more abundant in peripheral than central nervous system. Function: Gliomedin is a member of the collagen superfamily, it is a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes of ranvier. Eliminating the expression of gliomedin or the addition of a soluble extracellular domain of neurofascin to myelinating cultures abolishes node formation. Gliomedin is expressed in the PNS nodes of ranvier, but not in the CNS nodes of ranvier. Gliomedin also displays high expression in murine and human hepatocellular carcinomas (HCC). Its restricted expression in normal tissues and unique early upregulation during tumor development make it an excellent candidate as a new clinical marker of HCC. Subunit: Interacts with NFASC/neurofascin and NRCAM. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Note=Localizes to the nodes of Ranvier. Tissue Specificity: Specifically expressed in spinal cord, brain, placenta and sciatic nerve. More abundant in peripheral than central nervous system. Similarity: Contains 2 collagen-like domains. Contains 1 olfactomedin-like domain. SWISS: Q6ZMI3 Gene ID: 342035 Database links: Entrez Gene: 342035 Human Entrez Gene: 235379 Mouse Entrez Gene: 315675 Rat Omim: 608603 Human SwissProt: Q6ZMI3 Human SwissProt: Q7Z359 Human SwissProt: Q80ZC5 Mouse SwissProt: Q8BMF8 Mouse SwissProt: Q80WL1 Rat Unigene: 526441 Human Unigene: 123549 Mouse Unigene: 38054 Rat
Product Picture	Sample: B16(Mouse) Cell Lysate at 30 ug Primary: Anti-Gliomedin (SL11032R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD Sample: Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-Gliomedin (SL11032R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Gliomedin (SL11032R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gliomedin) Polyclonal Antibody, Unconjugated (SL11032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gliomedin) Polyclonal Antibody, Unconjugated (SL11032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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