Product Name	MID2
Chinese Name	Ring finger protein60抗体
Alias	FXY2; MID2; Midin-2; Midline 2; Midline defect 2; Midline-2; Probable E3 ubiquitin-protein ligase MID2; RING finger protein 60; Ring finger protein protein 60; RNF60; TRIM1; TRIM1_HUMAN; Tripartite motif protein 1; Tripartite motif-containing protein 1; Tripartite motif-containing protein 1; Midline defect 2; FLJ37715; FLJ41813.
Research Area	Neurobiology  Signal transduction  Cyclin  Cell adhesion molecule  Cytoskeleton  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	(predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	83kDa
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Midline-2: 251-350/735
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Midline-2 is a 715 amino acid protein encoded by the human gene MID2. Midline-2 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-2 is a cytoplasmic protein found as a homodimer or heterodimer with Midline-1. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Dimerization is mediated by the tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) and microtubule association is dependent on the C-terminal B30.2 domain. Midline-2 is expressed at low levels in fetal kidney and lung, and in adult prostate, ovary and small intestine. Subunit: Homodimer or heterodimer with MID1. Interacts with IGBP1. Tissue Specificity: Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine. Post-translational modifications: Phosphorylated on serine and threonine residues. Similarity: Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger. SWISS: Q9UJV3 Gene ID: 11043 Database links: Entrez Gene: 422131 Chicken Entrez Gene: 11043 Human Entrez Gene: 23947 Mouse Entrez Gene: 100157515 Pig Entrez Gene: 363502 Rat Omim: 300204 Human SwissProt: Q9UJV3 Human SwissProt: Q9QUS6 Mouse Unigene: 12256 Human Unigene: 131097 Mouse

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