Product Name	AFAP1L2
Chinese Name	AFAP1L2抗体
Alias	KIAA1914; XB130; Actin filament-associated protein 1-like 2; AF1L2_HUMAN; AFAP1-like protein 2; afap1l2.
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=2ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	90kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human AFAP1L2: 751-818/818
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Actin filament associated protein (AFAP-110) interacts directly with actin filaments through its C-terminal actin-binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. By linking signaling molecules to actin filaments, AFAP-110 provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. AFAP-1L2 (Actin filament-associated protein 1-like 2), also known as XB130, is a 818 amino acid cytoplasmic protein that contains two Pleckstrin homology (PH) domains, which are normally found in proteins involved in intracellular signaling. Like its relative AFAP110, AFAP-1L2 interacts with Src kinase and may play a role in Src-regulated transcription activation. AFAP-1L2 is expressed in thyroid and spleen and can also be detected at lower levels in lung, brain, pancreas and kidney. There are four isoforms of AFAP-1L2 that are produced as a result of alternative splicing events. Function: May play a role in a signaling cascade by enhancing the kinase activity of SRC. Contributes to SRC-regulated transcription activation. Subunit: Interacts with SRC. Interacts with LCK when tyrosine phosphorylated. Subcellular Location: Cytoplasm. Tissue Specificity: Detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas. Post-translational modifications: Tyrosine phosphorylated (by SRC). Similarity: Contains 2 PH domains. SWISS: Q8N4X5 Gene ID: 84632 Database links: Entrez Gene: 84632 Human Entrez Gene: 226250 Mouse Entrez Gene: 292130 Rat Omim: 612420 Human SwissProt: Q8N4X5 Human SwissProt: Q5DTU0 Mouse Unigene: 501106 Human Unigene: 226284 Mouse
Product Picture	Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- AFAP1L2 (SL11020R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 90 kD Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-AFAP1L2 antibody (SL11020R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Totalgoods,subtotals:￥Checkout