Product Name	phospho-IRS-2 (Ser731)
Chinese Name	磷酸化胰岛素受体底物2抗体
Alias	IRS-2(phospho-Ser731); IRS-2(phospho S731); Insulin Receptor Substrate 2; IRS 2; IRS-2; IRS2; IRS2_HUMAN.
literatures	Specific References  (1)     |     SL10998R has been referenced in 1 publications. [IF=2.868] Liu B et al. MicroRNA‐379 mediates pigmentation, migration, and proliferation of melanocytes by targeting the insulin‐like growth factor 1 receptor. Exp Dermatol. 2020 May;29(5):467-476.  WB ;  alpaca.   PubMed:32170969
Research Area	Cardiovascular  Cell biology  immunology  Neurobiology  Signal transduction  Growth factors and hormones  transcriptional regulatory factor  Diabetes
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Dog, Horse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	147kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human IRS-2 around the phosphorylation site of Ser731: AS(p-S)PA
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The family of insulin receptor substrates (IRSs) has been reported to play important roles for signal transduction of various hormones. Four members of the IRS family have been described. Each IRS is believed to have different functions; however, the distinct physiological roles of each IRS are unclear. Summary: This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. Function: May mediate the control of various cellular processes by insulin. Subunit: Interacts with PHIP. Subcellular Location: Cytoplasm, cytosol. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: Q9Y4H2 Gene ID: 8660 Database links: Entrez Gene: 8660 Human Entrez Gene: 384783 Mouse Entrez Gene: 29376 Rat Omim: 600797 Human SwissProt: Q9Y4H2 Human SwissProt: P81122 Mouse Unigene: 442344 Human Unigene: 407207 Mouse
Product Picture	Blank control (Black line): Raji (Black). Primary Antibody (red line): Rabbit Anti-Phospho-IRS-2(Ser731) antibody (SL10998R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (green line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA （10min）and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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