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Product Name phospho-Ephrin B2 (Tyr311/316) Chinese Name 磷酸化Ephrin B2抗体 Alias phospho-Ephrin B2 (Tyr311+Tyr316); Ephrin B2 (phospho Y311 + Y316); CEK5 ligand; EFNB1; ELK L; EphrinB; Ephrin-B; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B Precursor; Ephrin B1; Ephrin B2; LERK 2; LERK2; EFNB1_HUMAN; EFNB2_HUMAN. Research Area Tumour Cell biology immunology Neurobiology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:200-1000 ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 36kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human Ephrin B2 around the phosphorylation site of Tyr311/316: GD(p-Y)GHPV(p-Y)IV Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.
Subunit:
Interacts with GRIP1 and GRIP2. Binds to Nipah virus G protein.
Subcellular Location:
Membrane; single pass type I membrane protein.
Tissue Specificity:
Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Similarity:
Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain.
SWISS:
P52799
Gene ID:
1947
Database links:Entrez Gene: 1947 Human
Entrez Gene: 1948 Human
Entrez Gene: 13641 Mouse
Entrez Gene: 13642 Mouse
Omim: 300035 Human
Omim: 600527 Human
SwissProt: P52799 Human
SwissProt: P98172 Human
SwissProt: P52795 Mouse
SwissProt: P52800 Mouse
Unigene: 144700 Human
Unigene: 149239 Human
Unigene: 44398 Rat
Product Picture Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-phospho-Ephrin B2 (Tyr311/316) (SL10940R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
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