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Rabbit Anti-phospho-Ephrin B2 (Tyr311/316)antibody
Rabbit Anti-phospho-Ephrin B2 (Tyr311/316)antibody
phospho-Ephrin B2 (Tyr311+Tyr316); Ephrin B2 (phospho Y311 + Y316); CEK5 ligand; EFNB1; ELK L; EphrinB; Ephrin-B; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B Precursor; Ephrin B1; Ephrin B2; LERK 2; LERK2; EFNB1_HUMAN; EFNB2_
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  • NO.:SL10940R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,)
    Applications:WB ELISA IHC-F IF
    concentration:1mg/ml
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Product Name phospho-Ephrin B2 (Tyr311/316)
Chinese Name 磷酸化Ephrin B2抗体
Alias phospho-Ephrin B2 (Tyr311+Tyr316); Ephrin B2 (phospho Y311 + Y316); CEK5 ligand; EFNB1; ELK L; EphrinB; Ephrin-B; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B Precursor; Ephrin B1; Ephrin B2; LERK 2; LERK2; EFNB1_HUMAN; EFNB2_HUMAN.  
Research Area Tumour  Cell biology  immunology  Neurobiology  Signal transduction  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications WB=1:200-1000 ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 36kDa
Cellular localization The cell membrane 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated Synthesised phosphopeptide derived from human Ephrin B2 around the phosphorylation site of Tyr311/316: GD(p-Y)GHPV(p-Y)IV 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.

Subunit:
Interacts with GRIP1 and GRIP2. Binds to Nipah virus G protein.

Subcellular Location:
Membrane; single pass type I membrane protein.

Tissue Specificity:
Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.

Similarity:
Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain.

SWISS:
P52799

Gene ID:
1947

Database links:

Entrez Gene: 1947 Human

Entrez Gene: 1948 Human

Entrez Gene: 13641 Mouse

Entrez Gene: 13642 Mouse

Entrez Gene: 25186 Rat

Omim: 300035 Human

Omim: 600527 Human

SwissProt: P52799 Human

SwissProt: P98172 Human

SwissProt: P52795 Mouse

SwissProt: P52800 Mouse

SwissProt: P52796 Rat

SwissProt: Q6P7B6 Rat

Unigene: 144700 Human

Unigene: 149239 Human

Unigene: 44398 Rat



Product Picture
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-phospho-Ephrin B2 (Tyr311/316) (SL10940R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa

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