Product Name	MRP2/ABCC2
Chinese Name	多药耐药相关蛋白2抗体
Alias	multidrug resistance-associated protein2; ABC30; ABCC2; ATP binding cassette sub family C (CFTR/MRP) member 2; ATP binding cassette subfamily C member 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; CMOAT; CMOAT1; cMRP; DJS; KIAA1010; MRP 2; MRP-2; MRP2; Multidrug resistance associated protein 2; MRP2_HUMAN; ATP-binding cassette sub-family C member 2; Multidrug resistance-associated protein 2.
literatures	Specific References  (11)     |     SL1092R has been referenced in 11 publications. [IF=17.694] Tang Bo. et al. Gut microbiota alters host bile acid metabolism to contribute to intrahepatic cholestasis of pregnancy. NAT COMMUN. 2023 Mar;14(1):1-17  IHC-P ;  Mouse.   PubMed:36894566 [IF=7.149] Yuan P et al. WS2 Nanosheets at Noncytotoxic Concentrations Enhance the Cytotoxicity of Organic Pollutants by Disturbing the Plasma Membrane and Efflux Pumps. Environ Sci Technol. 2020 Feb 4;54(3):1698-1709.  WB ;  Mouse&Human.   PubMed:31916439 [IF=6.525] Silvia Di Giacomo. et al. Sorafenib Chemosensitization by Caryophyllane Sesquiterpenes in Liver, Biliary, and Pancreatic Cancer Cells: The Role of STAT3/ABC Transporter Axis. PHARMACEUTICS. 2022 Jun;14(6):1264  IF ;  Human.   PubMed:35745837 [IF=5.988] Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678  IHC, IF ;  Rat.   PubMed:35873589 [IF=5.195] Yilin Sun. et al. Rhus chinensis Mill. fruits alleviate liver injury induced by isoniazid and rifampicin through regulating oxidative stress, apoptosis, and bile acid transport. J ETHNOPHARMACOL. 2023 Jun;310:116387  WB ;  Mouse.   PubMed:36948265 [IF=4.407] Xin Fang. et al. Cigarette smoke extract combined with LPS down-regulates the expression of MRP2 in chronic pulmonary inflammation may be related to FXR. Mol Immunol. 2021 Sep;137:174  IHC ;  Rat.   PubMed:34273652 [IF=4.407] Caijun Zhao. et al. Kynurenic acid protects against mastitis in mice by ameliorating inflammatory responses and enhancing blood-milk barrier integrity. Mol Immunol. 2021 Sep;137:134  WB ;  Mouse.   PubMed:34247099 [IF=4.366] Shimeng Qiu. et al. An Efficient Method for the Differentiation of Human iPSC-Derived Endoderm toward Enterocytes and Hepatocytes. Cells-Basel. 2021 Apr;10(4):812  IF ;  Human.   PubMed:33917333 [IF=2.495] Yuge Liao. et al. Inorganic mercury induces liver oxidative stress injury in quails by inhibiting Akt/Nrf2 signal pathway. INORG CHEM COMMUN. 2022 Aug;142:109603  WB ;  Bird.   PubMed:10.1016/j.inoche.2022.109603 [IF=2.311] Haruna Makita. et al. Xenografts derived from patients with head and neck cancer recapitulate patient tumour properties. Oncol Lett. 2021 May;21(5):1-10  IHC ;  Human.   PubMed:33777208 [IF=2.295] Xin Fang. et al. The changes of MRP2 expression in three kinds of pulmonary inflammation models: the downregulation occurred in cigarette smoke extract (CSE) stimulation group and CSE plus LPS stimulation group, unchanged in LPS stimulation group. 2021 Mar 23  WB ;  Human.   PubMed:33752573
Research Area	Tumour
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	174kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MRP2: 485-615/1545 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	multidrug resistance-associated protein 2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia. Belongs to the ABC transporter family. Function: Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. DISEASE: Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function. Similarity: Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. SWISS: Q92887 Gene ID: 1244 Database links: Entrez Gene: 1244 Human Entrez Gene: 12780 Mouse Entrez Gene: 25303 Rat Omim: 601107 Human SwissProt: Q92887 Human SwissProt: Q8VI47 Mouse SwissProt: Q63120 Rat Unigene: 368243 Human Unigene: 39054 Mouse Unigene: 10265 Rat MRP-2作为一种结合输出泵，转运许多不同的药物结合物的蛋白。 MRP2蛋白的染色阳性产物分布于cytoplasmic/膜中。
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-ABCC2 (SL1092R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 174 kD Observed band size: 174 kD Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: A549 (Human) Cell Lysate at 30 ug Lane 3: Panc-1 (Human) Cell Lysate at 30 ug Primary: Anti-MRP2/ABCC2 (SL1092R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 180 kD Observed band size: 180 kD Sample: HepG2(Human) Cell Lysate at 40 ug Primary: Anti- MRP2/ABCC2 (SL1092R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 174 kD Observed band size: 174 kD Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MRP2/ABCC2) Polyclonal Antibody, Unconjugated (SL1092R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABCC2) Polyclonal Antibody, Unconjugated (rat kidney) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRP2 Polyclonal Antibody, Unconjugated(SL1092R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human liver carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRP2 Polyclonal Antibody, Unconjugated(SL1092R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(SL0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. Blank control: A549. Primary Antibody (green line): Rabbit Anti-ABCC2 antibody (SL1092R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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