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Product Name Myosin-3 Chinese Name 肌球蛋白3抗体 Alias MYH3_HUMAN; Myosin-3; Muscle embryonic myosin heavy chain; Myosin heavy chain 3; Myosin heavy chain, fast skeletal muscle, embryonic; SMHCE. literatures Specific References (2) | SL10905R has been referenced in 2 publications.[IF=8.702] Xiao, Jiangwei. et al. IRE1α arm of unfolded protein response in muscle-specific TGF-β signaling-mediated regulation of muscle cell immunological properties. CELL MOL BIOL LETT. 2023 Dec;28(1):1-24 IF ; Mouse.[IF=3.329] Yin W et al. Guanine-rich RNA binding protein GRSF1 inhibits myoblast differentiation through repressingmitochondrial ROS production. Exp Cell Res. 2019 Aug 1;381(1):139-149. ICF ; Mouse.Research Area Cell biology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Cow, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 224kDa Cellular localization cytoplasmic Extracellular matrix Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Myosin-3: 1801-1940/1940 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Myosin-3 Polyclonal Antibody, Unconjugated(SL10905R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat skeletal muscle; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Myosin-3 Polyclonal Antibody, Unconjugated(SL10905R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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