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Product Name NGFB Chinese Name 神经生长因子β抗体 Alias NGF; Beta nerve growth factor; NGF-beta; Beta nerve growth factor precursor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor beta; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGF B; NGFB; NID67; NGF beta; NGF-B; NGF_HUMAN. literatures Specific References (1) | SL10806R has been referenced in 1 publications.[IF=1.894] Zhou M et al. Cardiac Sympathetic Afferent Denervation Protects Against Ventricular Arrhythmias by Modulating Cardiac Sympathetic Nerve Activity During Acute Myocardial Infarction.Med Sci Monit. 2019 Mar 16;25:1984-1993. WB ; Dog.Research Area Developmental biology Neurobiology cell factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 13/32kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen Recombinded human NGF beta full length protein Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]
Function:
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.
Subunit:
Homodimer.
Subcellular Location:
Secreted.
DISEASE:
Defects in NGF are the cause of hereditary sensory and autonomic neuropathy type 5 (HSAN5) [MIM:608654]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.
Similarity:
Belongs to the NGF-beta family.
SWISS:
P01138
Gene ID:
4803
Database links:Entrez Gene: 4803 Human
Entrez Gene: 18049 Mouse
Omim: 162030 Human
SwissProt: P01138 Human
SwissProt: P01139 Mouse
Unigene: 2561 Human
Unigene: 1259 Mouse
Unigene: 22168 Rat
Product Picture Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Skin (Mouse) Lysate at 40 ug
Lane 3: Thyroid gland (Mouse) Lysate at 40 ug
Primary: Anti-NGFB (SL10806R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32/13 kD
Observed band size: 35/13 kD
Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Human U-87 MG cell Lysates
Lane 4: Human SH-SY5Y cell Lysates
Lane 5: Human U-2 OS cell Lysates
Primary: Anti-NGFB (SL10806R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/32kDa
Observed band size: 13/32kDa
Sample:
Brain (Rat) Lysate at 40 ug
Primary: Anti-NGFB (SL10806R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13 kD
Observed band size: 15 kD
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NGFB Polyclonal Antibody, Unconjugated(SL10806R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (SL10806R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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