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Product Name NUP88 Chinese Name 核孔复合体蛋白88抗体 Alias karyoporin; Nuclear pore complex protein 88; Nuclear pore complex protein88; Nucleoporin 88; Nucleoporin 88 kDa; Nucleoporin 88kDa; Nucleoporin88; Nucleoporin88kDa; Nup 84; NUP 88; Nup84; Prei 2; Prei2; Preimplantation protein 2; Preimplantation protein2; NUP88_HUMAN. Research Area Tumour Chromatin and nuclear signals Signal transduction TumourCell biologyMaker Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 85kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NUP88: 551-650/741 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NUP88 is a nucleoporin that is an essential component of the nuclear pore complex. It interacts with NUP214/CAN, and is phosphorylated following DNA damage, possibly via the ATM/ATR pathway. NUP88 is also a tumour marker as it is overexpressed in a large number of malignant neoplasms and precancerous dysplasias.
Function:
Essential component of nuclear pore complex.
Subunit:
Interacts with NUP214/CAN.
Subcellular Location:
Nucleus, nuclear pore complex.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
SWISS:
Q99567
Gene ID:
4927
Database links:Entrez Gene: 4927 Human
Entrez Gene: 19069 Mouse
Omim: 602552 Human
SwissProt: Q99567 Human
SwissProt: Q8CEC0 Mouse
Unigene: 584784 Human
Unigene: 288962 Mouse
Unigene: 11042 Rat
Product Picture Sample:
testis (Mouse) Lysate at 40 ug
Primary: Anti-NUP88 (SL10782R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD
Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUP88) Polyclonal Antibody, Unconjugated (SL10782R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUP88) Polyclonal Antibody, Unconjugated (SL10782R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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