Product Name	Nrf2
Chinese Name	核因子2相关因子2抗体
Alias	Nuclear factor-E2 related factor2; Nrf-2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN.
literatures	Specific References  (123)     |     SL1074R has been referenced in 123 publications. [IF=10.183] Jae Yeon Kim. et al. Increased PRL-1 in bone marrow-derived mesenchymal stem cells triggers anaerobic metabolism via mitochondria in a cholestatic rat model. MOL THER-NUCL ACIDS. 2023 Feb 04  WB ;  Rat.   PubMed:36865088 [IF=8.724] Jianmei Li. et al. Mn-containing bioceramics inhibit osteoclastogenesis and promote osteoporotic bone regeneration via scavenging ROS. Bioact Mater. 2021 Nov;6:3839  IF ;  Rat.   PubMed:33898880 [IF=8.44] Li Xiangdong. et al. Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3. J TRANSL MED. 2023 Dec;21(1):1-19  WB ;  Mouse.   PubMed:36788538 [IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268.  WB ;  Human.   PubMed:doi:10.1016/j.cej.2020.124268 [IF=7.675] Hee Jung Park. et al. Phosphatase of Regenerating Liver-1 (PRL-1)-Overexpressing Placenta-Derived Mesenchymal Stem Cells Enhance Antioxidant Effects via Peroxiredoxin 3 in TAA-Injured Rat Livers. ANTIOXIDANTS-BASEL. 2023 Jan;12(1):46  WB ;  Rat.   PubMed:36670907 [IF=7.675] Shuang Wang. et al. Dioscin Protects against Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling. ANTIOXIDANTS-BASEL. 2022 Dec;11(12):2443  IF ;  Rat.   PubMed:36552651 [IF=7.675] Xiaoxiao Zou. et al. Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice. ANTIOXIDANTS-BASEL. 2023 Mar;12(3):712  WB ;  Mouse.   PubMed:36978962 [IF=7.129] Yuan-yuan Wei. et al. Acute liver injury induced by carbon tetrachloride reversal by Gandankang aqueous extracts through nuclear factor erythroid 2-related factor 2 signaling pathway. ECOTOX ENVIRON SAFE. 2023 Feb;251:114527  IHC ;  Mouse.   PubMed:36628874 [IF=7.129] Rui Sang. et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. ECOTOX ENVIRON SAFE. 2023 Feb;251:114546  WB ;  Chicken.   PubMed:36646010 [IF=7.129] Jie Luo. et al. Copper nanoparticles lead to reproductive dysfunction by affecting key enzymes of ovarian hormone synthesis and metabolism in female rats. ECOTOX ENVIRON SAFE. 2023 Apr;254:114704  WB ;  Rat.   PubMed:36898311 [IF=7.086] Xue Yang. et al. The heart as a target for deltamethrin toxicity: Inhibition of Nrf2/HO-1 pathway induces oxidative stress and results in inflammation and apoptosis. Chemosphere. 2022 Aug;300:134479  WB ;  Japanese Quails.   PubMed:35367492 [IF=6.953] Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453  WB ;  Human.   PubMed:35339497 [IF=6.832] Tao Cai. et al. Long noncoding RNA BBOX1-AS1 promotes the progression of gastric cancer by regulating the miR-361-3p/Mucin 13 signaling axis. BIOENGINEERED. 2022 Jun 05  WB ;  Human.   PubMed:10.1080/21655979.2022.2072629 [IF=6.792] Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319  WB ;  Quail.   PubMed:33990053 [IF=6.543] Zhong Li. et al. The TBX1/miR-193a-3p/TGF-β2 Axis Mediates CHD by Promoting Ferroptosis. Oxid Med Cell Longev. 2022;2022:5130546  WB ;  Human.   PubMed:35035663 [IF=6.543] Deng Liangjun. et al. The Attenuation of Chronic Ulcerative Colitis by (R)-salbutamol in Repeated DSS-Induced Mice. Oxid Med Cell Longev. 2022;2022:9318721  WB,IF ;  Mouse.   PubMed:35178163 [IF=6.543] Wang Da-Peng. et al. URB597 and Andrographolide Improve Brain Microvascular Endothelial Cell Permeability and Apoptosis by Reducing Oxidative Stress and Inflammation Associated with Activation of Nrf2 Signaling in Oxygen-Glucose Deprivation. OXID MED CELL LONGEV. 2022;2022:4139330  WB ;  Mouse.   PubMed:35602108 [IF=6.543] Yuan Jiyan. et al. Vitamin B12 Attenuates Acute Pancreatitis by Suppressing Oxidative Stress and Improving Mitochondria Dysfunction via CBS/SIRT1 Pathway. Oxid Med Cell Longev. 2021;2021:7936316  WB ;  Mouse.   PubMed:34925701 [IF=6.344] Xin, Liangjing. et al. Four-Octyl itaconate ameliorates periodontal destruction via Nrf2-dependent antioxidant system. INT J ORAL SCI. 2022 May;14(1):1-12  IHC ;  Mouse.   PubMed:35637195 [IF=6.291] Xiao Wang. et al. Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis. Ecotox Environ Safe. 2022 Mar;233:113339  WB ;  Chicken.   PubMed:35219255 [IF=6.291] Xi Su. et al. Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats. Ecotox Environ Safe. 2021 Dec;225:112806  WB ;  Rat.   PubMed:34555716 [IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965  WB ;  Duck.   PubMed:34775344 [IF=6.208] Shuang Wang. et al. Paricalcitol Ameliorates Acute Kidney Injury in Mice by Suppressing Oxidative Stress and Inflammation via Nrf2/HO-1 Signaling. INT J MOL SCI. 2023 Jan;24(2):969  IF ;  Mouse.   PubMed:36674485 [IF=6.208] Yuya Otaka. et al. 5-ALA/SFC Ameliorates Endotoxin-Induced Ocular Inflammation in Rats by Inhibiting the NF-κB Signaling Pathway and Activating the HO-1/Nrf2 Signaling Pathway. INT J MOL SCI. 2023 Jan;24(10):8653  IHC ;  Rat.   PubMed:37239995 [IF=6.117] Yuanyuan Xing. et al. Artemisia ordosica polysaccharide ameliorated LPS-induced growth inhibition and intestinal injury in broilers through enhancing immune-regulation and antioxidant capacity. J NUTR BIOCHEM. 2023 Feb;:109284  WB ;  Chicken.   PubMed:36828238 [IF=5.81] Xinyue Chang. et al. The Protective Effect of Trichilia catigua A. Juss. on DEHP-Induced Reproductive System Damage in Male Mice. Front Pharmacol. 2022; 13: 832789  IHC ;  Mouse.   PubMed:35185586 [IF=5.589] Lv Y et al. Imidacloprid-induced liver fibrosis in quails via activation of the TGF-β1/Smad pathway. Sci Total Environ. 2019 Dec 6;705:135915.  WB ;  Quail.   PubMed:31835194 [IF=5.552] Huang, Jianjia. et al. Lycium barbarum polysaccharides ameliorate canine acute liver injury by reducing oxidative stress, protecting mitochondrial function, and regulating metabolic pathways. J ZHEJIANG UNIV-SC B. 2023 Feb;24(2):157-171  WB ;  Dog.   PubMed:36751701 [IF=5.34] Qingwen Hu. et al. β-Caryophyllene suppresses ferroptosis induced by cerebral ischemia reperfusion via activation of the NRF2/HO-1 signaling pathway in MCAO/R rats. PHYTOMEDICINE. 2022 Jul;102:154112  WB ;  Rat.   PubMed:35550220 [IF=5.27] Lee, Bao-Hong, et al. "Dimerumic acid attenuates receptor for advanced glycation endproduct (RAGE) signal to inhibit inflammation and diabetes mediated by Nrf2 activation and promoted methylglyoxal metabolism into D-lactate acid" Free Radical Biology and Medicine  WB ;  Human.   PubMed:23434766
Research Area	Tumour  immunology  Chromatin and nuclear signals  Synthesis and Degradation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Cow, Horse, Rabbit, )
Applications	WB=1:100-1000 ELISA=1:1000-5000 IHC-P=1:100-1000 IHC-F=1:100-1000 ICC=1:100 IF=1:100-1000 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	68kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Nrf2: 401-500/605
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Subunit: Heterodimer. May bind DNA with an unknown protein. Interacts with KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. Tissue Specificity: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. Post-translational modifications: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain. SWISS: Q16236 Gene ID: 4780 Database links: Entrez Gene: 4780 Human Omim: 600492 Human SwissProt: Q16236 Human transcriptional regulatory factor（Transcriptin Regulators） 核细胞系因子2相关因子Nrf2，是一种对氧化还原反应敏感的转录因子，可以通过正向促进基因的表达，来转录翻译产生一些抗氧化物，行使着异化解毒的酶类和药物的高效外排泵作用。 有学者认为：核因子2相关因子2蛋白在保护细胞免受环境毒性物质影响方面发挥着极其重要的作用，它指导某些基因促进细胞的防御工事，同时也刺激某些重要解毒酶对毒性物质，如黄曲霉素的解毒和排毒作用。
Product Picture	Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Liver ((Rat) Lysate at 40 ug Lane 4: SiHa (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Nrf2 (SL1074R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110’68 kD Observed band size: 110 kD Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074 R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse trachea); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse trachea); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074 R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (SL1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. 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