Product Name	NELL1
Chinese Name	蛋白激酶CBinding proteinNELL1抗体
Alias	IDH3GL; NEL like protein 1; Nel related protein 1; NEL-like protein 1; Nel-related protein 1; Nell1; NELL1_HUMAN; Neural epidermal growth factor like 1; NRP1; Protein kinase C binding protein NELL1; Protein kinase C-binding protein NELL1.
Research Area	Cell biology  Neurobiology  Signal transduction  Growth factors and hormones  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	87kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NELL1: 201-300/810
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Function: Involved in the control of cell growth and differentiation. Subunit: Homotrimer. Binds to PKC beta-1. Interacts with ATRAID; the interaction promotes osteoblast cell differentiation and mineralization. Subcellular Location: Secreted. Similarity: Contains 6 EGF-like domains. Contains 1 TSP N-terminal (TSPN) domain. Contains 5 VWFC domains. SWISS: Q92832 Gene ID: 4745 Database links: Entrez Gene: 4745 Human Entrez Gene: 338352 Mouse Entrez Gene: 81733 Rat Omim: 602319 Human SwissProt: Q92832 Human SwissProt: Q2VWQ2 Mouse SwissProt: Q62919 Rat Unigene: 657172 Human Unigene: 383528 Mouse Unigene: 10695 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL1) Polyclonal Antibody, Unconjugated (SL10704R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL1) Polyclonal Antibody, Unconjugated (SL10704R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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