Product Name	CD8
Chinese Name	CD8抗体
Alias	CD8 antigen, alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen.
literatures	Specific References  (4)     |     SL10699R has been referenced in 4 publications. [IF=3.454] Chong Zhang. et al. Histological tumor response to neoadjuvant chemotherapy correlates to Immunoscore in colorectal cancer liver metastases patients. 2021 Aug 18  IHC ;  Human.   PubMed:34406653 [IF=3.223] Shifa Zhang. et al. Impact of the combination of sintilimab and chemotherapy on the tumor and paratumor PD-L1, IDO, TIM-3, FOXP3+ and CD8 expressions in patients with advanced esophageal squamous cell carcinoma. THORAC CANCER. 2022 Oct;:  IHC ;  Human.   PubMed:36288460 [IF=1.664] Song et al. Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine on metastatic breast cancer in a mouse model. (2018) Oncol.Lett. 15:6831-6838  IHC ;  mouse.   PubMed:29731862 [IF=1.664] Song N et al. Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine onmetastatic breast cancer in a mouse model. Oncology Letters.2018.  IHC-P ;  Mouse.   PubMed:10.3892/ol.2018.8188
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	27kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD8: 101-200/247 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]. Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P01731 Gene ID: 12525 Database links: Entrez Gene: 925 Human Entrez Gene: 926 Human Entrez Gene: 12525 Mouse Entrez Gene: 12526 Mouse Omim: 186910 Human SwissProt: P01732 Human SwissProt: P10966 Human SwissProt: P01731 Mouse SwissProt: P10300 Mouse Unigene: 405667 Human Unigene: 85258 Human Unigene: 1858 Mouse Unigene: 333148 Mouse
Product Picture	Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Lymph node tissue lysates Lane 3: Rat Thymus tissue lysates Primary: Anti-CD8 (SL10699R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kDa Observed band size: 45 kDa Blank control (blue line): Mouse Spleen cells(fixed with 70% methanol (Overnight at 4℃)). Primary Antibody (green line): Rabbit Anti-CD8 antibody (SL10699R),Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. Blank control: Mouse splen. Primary Antibody (green line): Rabbit Anti-CD8 antibody (SL10699R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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