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Product Name SRGAP1 Chinese Name Rho GTP酶激活蛋白13抗体 Alias ARHGAP13; FLJ22166; KIAA1304; RGD1566260; Rho GTPase activating protein 13; SLIT ROBO Rho GTPase activating protein 1; SRGAP 1; SRGP1_HUMAN. Research Area Cell biology Neurobiology Signal transduction G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 124kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SRGAP1: 51-150/1085 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SRGAPs contain a highly conserved overall primary structure and play an important role in the cell facilitating Slit-robo signaling in cell migration and axon guidance. SRGAP1 (Slit-robo Rho GTPase activating protein 1), also known as ARHGAP13 (Rho GTPase activating protein 13), functions as a GTPase-activating protein for Cdc42 and Rho A. Expressed in kidney, testis, lung and brain, SRGAP1 contains an FCH (Fes/CIP4 homology) domain, a Rho-GAP domain and an SH3 domain. In the presence of Slit, SRGAP1 (via its SH3 domain) binds to the CC3 motif in robo (a protein responsible for mediating the repulsive effect of Slit) with higher affinity and inhibits Cdc42 activity in a robo/SRGAP-dependent manner. More specifically, SRGAP1 increases the intrinsic GTPase activity of Cdc42, thereby converting it to its inactive, GDP-bound form. Inactivation of Cdc42 ultimately leads to a decrease in actin polymerization.
Function:
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
Subunit:
Homodimer (Probable). Forms heterooligomer with SRGAP2 and SRGAP3 through its F-BAR domain. Interacts with ROBO1, CDC42 and RHOA. Interacts with FASLG.
Tissue Specificity:
Expressed in brain, lung, kidney, and testis.
Similarity:
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
SWISS:
Q7Z6B7
Gene ID:
57522
Database links:Entrez Gene: 57522 Human
Entrez Gene: 117600 Mouse
Omim: 606523 Human
SwissProt: Q7Z6B7 Human
SwissProt: Q91Z69 Mouse
Unigene: 210751 Human
Unigene: 593803 Human
Unigene: 288698 Mouse
Unigene: 208903 Rat
Product Picture Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SRGAP1 Polyclonal Antibody, Unconjugated(SL10671R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SRGAP1 Polyclonal Antibody, Unconjugated(SL10671R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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