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Product Name PI3 Kinase p110 delta Chinese Name 磷脂酰肌醇激酶催化亚单位D抗体 Alias p110D; p110delta; Phosphatidylinositol 3 kinase catalytic delta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphoinositide 3 kinase B; Phosphoinositide 3 kinase C; Phosphoinositide 3 kinase catalytic delta polypeptide; PI3 kinase p110 subunit delta; PI3K; PIK3CD; PK3CD; PtdIns 3 kinase p110; PI 3 Kinase p110 delta; PK3CD_HUMAN; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; PI3-kinase subunit delta; PI3K-delta; PI3Kdelta; PtdIns-3-kinase subunit delta; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; PtdIns-3-kinase subunit p110-delta; p110delta. literatures Specific References (2) | SL10656R has been referenced in 2 publications.[IF=6.656] Fangbo Zhang. et al. Huashibaidu Formula Attenuates Sepsis-Induced Acute Lung Injury via Suppressing Cytokine Storm: Implications for Treatment of COVID-19. PHYTOMEDICINE. 2022 Nov;:154549 WB ; Mouse.[IF=5.561] Minrui Wu. et al. The Effect and Mechanism of Corilagin from Euryale Ferox Salisb Shell on LPS-Induced Inflammation in Raw264.7 Cells. FOODS. 2023 Jan;12(5):979 WB ; Mouse.Research Area Tumour immunology Signal transduction transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 115kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PI3 Kinase p110 delta: 51-150/1044 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Sample: Thymus (Mouse) Lysate at 40 ug
Primary: Anti-PI3 Kinase p110 delta (SL10656R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Sample:
Lane 1: Human HT-1080 cell lysates
Lane 2: Human Jurkat cell lysates
Primary: Anti-PI3 Kinase p110 delta (SL10656R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 120 kDa
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PI3 Kinase p110 delta Polyclonal Antibody, Unconjugated(SL10656R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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