Product Name	ADRB3
Chinese Name	β3肾上腺素能受体抗体
Alias	beta 3 Adrenergic Receptor; beta3-adrenergic receptor; beta3-AR; adrenergic; beta-3- receptor; ADR B3; ADRB 3; ADRB3R; Adrenergic beta 3 receptor; B3AR; Beta 3 adrenoceptor; Beta 3 adrenoreceptor; Beta 3AR; B3AR; BETA3AR; ADRB3_HUMAN.
literatures	Specific References  (7)     |     SL1063R has been referenced in 7 publications. [IF=6.208] Lina S. Farhoumand. et al. Blockade of ß-Adrenergic Receptors by Nebivolol Enables Tumor Control Potential for Uveal Melanoma in 3D Tumor Spheroids and 2D Cultures. INT J MOL SCI. 2023 Jan;24(6):5894  FCM ;  Human.   PubMed:36982966 [IF=4.757] Katarina Andelova. et al. Cardiac Cx43 Signaling Is Enhanced and TGF-β1/SMAD2/3 Suppressed in Response to Cold Acclimation and Modulated by Thyroid Status in Hairless SHRM. BIOMEDICINES. 2022 Jul;10(7):1707  WB ;  Rat.   PubMed:35885012 [IF=4.699] Pellegrino Lippiello. et al. Role of β3‐adrenergic receptor in the modulation of synaptic transmission and plasticity in mouse cerebellar cortex. J Neurosci Res. 2020 Nov;98(11):2263-2274  IF ;  Mouse.   PubMed:33174240 [IF=3.647] Clarissa Germano Barp et al. Perivascular adipose tissue phenotype and sepsis vascular dysfunction: Differential contribution of NO, ROS and beta 3-adrenergic receptor. Life Sci. 2020 Aug 1;254:117819.  IF ;  Rat.   PubMed:32442451 [IF=3.14] Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.  WB ;  rat.   PubMed:32213114 [IF=2.705] Yamashita K et al. Adrenal cortex hypoxia modulates aldosterone production in heart failure. Biochem Biophys Res Commun. 2020 Jan 23.  IHC-P ;  Rat&Human.   PubMed:31982132 [IF=1.588] Tanaka M et al. Effect of mirabegron on tight junction molecules in primary cultured rat Sertoli cells. Andrologia. 2019 Jan 31:e13241.  ICC ;  Rat.   PubMed:30706522
Research Area	Cardiovascular  Cell biology  Neurobiology  Growth factors and hormones  The cell membrane受体  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ADRB3: 310-408/408 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein. Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed mainly in adipose tissues. Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily. SWISS: P25962 Gene ID: 155 Database links: Entrez Gene: 155 Human Entrez Gene: 11556 Mouse Entrez Gene: 25645 Rat SwissProt: P13945 Human SwissProt: P25962 Mouse SwissProt: P26255 Rat Unigene: 2549 Human Unigene: 278475 Mouse Unigene: 10100 Rat Omim: 109691 Human ARβ3是新近发现的,继β1和β2之后的第3个β型肾上腺素受体亚型.分子量：44KDa(404AA) 近年来研究表明:β3-肾上腺素受体该受体基因突变或基因多态性与机体肥胖及Cardiovascular疾病相关;心力衰竭时该受体的含量明显增加,β3肾上腺素能受体与Cardiovascular疾病的相关研究可能为Cardiovascular疾病的预测、防治的新方法.
Product Picture	Sample: K562(Human) Cell Lysate at 40 ug Primary: Anti-beta 3 Adrenergic Receptor (SL1063R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 54 kD Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated(SL1063R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Hela. Primary Antibody (green line): Rabbit Anti-beta 3 Adrenergic Receptor antibody (SL1063R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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