Product Name	MPO
Chinese Name	髓过氧化物酶抗体
Alias	Myeloperoxidase; c-ANCA; 89 kDa myeloperoxidase; 84 kDa yeloperoxidase; Myeloperoxidase light chain; Myeloperoxidase heavy chain; EC 1.11.1.7; PERM_HUMAN.
literatures	Specific References  (4)     |     SL1061R has been referenced in 4 publications. [IF=17.694] Zhang, Shengchang. et al. Remodeling articular immune homeostasis with an efferocytosis-informed nanoimitator mitigates rheumatoid arthritis in mice. NAT COMMUN. 2023 Feb;14(1):1-16  IHC ;  Mouse.   PubMed:36781864 [IF=6.064] Zhang Xuyang. et al. A modified method for constructing experimental rat periodontitis model. FRONT BIOENG BIOTECH. 2022 Dec;10:2451  IHC ;  Rat.   PubMed:10.3389/fbioe.2022.1098015 [IF=4.86] Li et al. Inhibition of p38/Mk2 signaling pathway improves the anti-inflammatory effect of WIN55 on mouse experimental colitis. (2013) Lab.Inves. 93:322-33  IHC ;  Mouse.   PubMed:23381627 [IF=2.05] Neupane S et al.Focal localization of inflammatory cytokines and neurotrophins in a tongue chronic injury model. (2019) Archives of Oral Biology 99, 22–30.  IHC-P ;  Mouse.   PubMed:30590230
Research Area	Tumour  Cell biology  immunology  Kinases and Phosphatases  lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	84kDa
Detection molecular weight	15/55-60/84-90 kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Myeloperoxidase: 51-150/745
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Subunit: Tetramer of two light chains and two heavy chains. Subcellular Location: Lysosome. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. Similarity: Belongs to the peroxidase family. XPO subfamily. SWISS: P05164 Gene ID: 4353 Database links: Entrez Gene: 4353 Human Entrez Gene: 17523 Mouse Entrez Gene: 303413 Rat Omim: 606989 Human SwissProt: P05164 Human SwissProt: P11247 Mouse Unigene: 458272 Human Unigene: 4668 Mouse Unigene: 47782 Rat 髓过氧化物酶MPO,作为一种白细胞酶,具有介导炎性反应、调节免疫应答等多种功能,并可参与疾病的发生发展过程。同时,髓过氧化物酶基因存在基因多态性,也影响机体对疾病的易感性. 在正常淋巴组织中和各种髓样细胞增生症中，MPO均有较强表达，如：淋巴样细胞、原核细胞、肥大细胞、浆细胞以及各种上皮源性Tumour和肉瘤等。 髓过氧化物酶(myeloperoxidase,MPO),是一种血红素蛋白，富含于中性粒细胞中,由粒细胞进入循环之前在骨髓内合成并存储于噬天青颗粒内。外界刺激可导致中性粒细胞聚集，从而释放髓过氧化物酶。MPO的相对分子量为150kDa，是由两个亚单位通过共价结合形成的四聚体，每个亚单位又有一条重链α（相对分子量60kDa）和一条轻链β链（相对分子量为15kDa)构成。（重链和轻链通过二硫键结合）
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- Myeloperoxidase (SL1061R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 63 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Myeloperoxidase) Polyclonal Antibody, Unconjugated (SL1061R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Myeloperoxidase Polyclonal Antibody, Unconjugated(SL1061R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MPO/c-ANCA Polyclonal Antibody, Unconjugated(SL1061R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Myeloperoxidase antibody (SL1061R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-Myeloperoxidase antibody (SL1061R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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