Product Name	Cytokeratin 5
Chinese Name	细胞角蛋白5抗体
Alias	Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; CK 5; CK5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; DDD; EBS 2; EBS2; K5; Keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5; Cytokeratin-5; Cytokeratin5; DDD; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; Keratin 5; Keratin; Keratin Type II Cytoskeletal 5; Keratin5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5.
literatures	Specific References  (8)     |     SL1060R has been referenced in 8 publications. [IF=10.269] Zhang, Xuefei. et al. A multifunctional cholesterol-free liposomal platform based on protopanaxadiol for alopecia therapy. NANO RES. 2022 Jul;:1-13  IF ;  Mouse.   PubMed:10.1007/s12274-022-4710-y [IF=8.077] Wang Liying. et al. SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity. J CELL BIOL. 2022 Jul;221(7):e202108015.   PubMed:35674692 [IF=8.025] Xinxin Lan. et al. Xanthan gum/oil body-microgel emulsions with enhanced transdermal absorption for accelerating wound healing. INT J BIOL MACROMOL. 2022 Sep;:  IF, WB ;  Mouse.   PubMed:36126813 [IF=5.875] Jie Kong. et al. Safflower oil body nanoparticles deliver hFGF10 to hair follicles and reduce microinflammation to accelerate hair regeneration in androgenetic alopecia. Int J Pharmaceut. 2022 Mar;616:121537  IF ;  Mouse.   PubMed:35150848 [IF=4.556] Yu X et al. Transdermal Cubic Phases of Metformin Hydrochloride: In Silico and In Vitro Studies of Delivery Mechanisms.Mol Pharm. 2018 Aug 6;15(8):3121-3132.  IHC-F ;  Mouse.   PubMed:29979604 [IF=3.591] Zhengrong Yin. et al. Dissolvable and layered microneedles composed of hyaluronate/rbFGF/CPC effectively improve the treatment effect on recurrent aphthous ulcers. New J Chem. 2022 Mar;:  IF ;  Rat.   PubMed:10.1039/D2NJ00054G [IF=3.011] Boyu Chen. et al. Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking. Exp Eye Res. 2021 Jul;:108695  ICC ;  Rabbit.   PubMed:34228966 [IF=1.329] Ziwei Luo. et al. miR‐203a‐3p promotes loureirin A‐induced hair follicle stem cells differentiation by targeting Smad1. Anat Rec. 2021 Mar;304(3):531-540  IF ;  Rat.   PubMed:32589363
Research Area	Tumour  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	64kDa
Cellular localization	The nucleus cytoplasmic Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CK5: 331-430/590
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex. Subunit: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. DISEASE: Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. Defects in KRT5 are the cause of epidermolysis bullosa simplex with migratory circinate erythema (EBSMCE) [MIM:609352]. EBSMCE is a form of intraepidermal epidermolysis bullosa characterized by unusual migratory circinate erythema. Skin lesions appear from birth primarily on the hands, feet, and legs but spare nails, ocular epithelia and mucosae. Lesions heal with brown pigmentation but no scarring. Electron microscopy findings are distinct from those seen in the DM-EBS, with no evidence of tonofilament clumping. Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin. Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe. Defects in KRT5 are the cause of epidermolysis bullosa simplex with mottled pigmentation (MP-EBS) [MIM:131960]. MP-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering at acral sites and 'mottled' pigmentation of the trunk and proximal extremities with hyper- and hypopigmentation macules. Defects in KRT5 are the cause of Dowling-Degos disease (DDD) [MIM:179850]; also known as Dowling-Degos-Kitamura disease or reticulate acropigmentation of Kitamura. DDD is an autosomal dominant genodermatosis. Affected individuals develop a postpubertal reticulate hyperpigmentation that is progressive and disfiguring, and small hyperkeratotic dark brown papules that affect mainly the flexures and great skin folds. Patients usually show no abnormalities of the hair or nails. Similarity: Belongs to the intermediate filament family. SWISS: P13647 Gene ID: 3852 Database links: Entrez Gene: 3852 Human Entrez Gene: 110308 Mouse Entrez Gene: 369017 Rat Omim: 148040 Human SwissProt: P13647 Human SwissProt: Q922U2 Mouse SwissProt: Q6P6Q2 Rat Unigene: 433845 Human Unigene: 451847 Mouse Unigene: 129725 Rat Unigene: 195318 Rat 结构蛋白（Structural Proteins） 细胞角蛋白5，为高分子量细胞角蛋白 58 kDa ，表达在皮肤的基底细胞和棘层细胞，部分前列腺基底细胞，与其它单层腺上皮不表达。主要用于间皮瘤与腺癌的鉴别诊断。 细胞角蛋白是一类与结构相关的蛋白家族，其在epithelial cells中形成Cytoskeleton中间丝。CK5是在表皮角质化细胞中大量表达的4种角蛋白之一。CK5可用以区分正常细胞和Tumour细胞。在基底细胞上皮瘤、多种鳞状细胞癌（皮肤和舌）、多种epithelial cells和间皮瘤都有CK5的表达。
Product Picture	Sample: Skin (Mouse) Lysate at 40 ug Primary: Anti-Cytokeratin 5 (SL1060R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: MDA-MB-231(Human) Cell Lysate at 40 ug MCF-7(Human) Cell Lysate at 40 ug Primary: Anti-Cytokeratin 5 (SL1060R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 64 kD Observed band size: 64 kD Sample: Lane 1: Skin (Mouse) Lysate at 40 ug Lane 2: Uterus (Mouse) Lysate at 40 ug Lane 3: Urinary bladder (Mouse) Lysate at 40 ug Lane 4: Esophagus (Mouse) Lysate at 40 ug Lane 5: Skin (Rat) Lysate at 40 ug Lane 6: Uterus (Rat) Lysate at 40 ug Lane 7: Urinary bladder (Rat) Lysate at 40 ug Lane 8: Esophagus (Rat) Lysate at 40 ug Primary: Anti-Cytokeratin 5 (SL1060R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (SL1060R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (SL1060R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat bladder tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (SL1060R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control: Hela Cells(blue). Primary Antibody: Rabbit Anti-Cytokeratin 5/AF488 Conjugated antibody (SL1060R/AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 (orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (SL1060R/AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

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