Product Name	IGFBP4
Chinese Name	胰岛素样生长因子Binding protein4抗体
Alias	Insulin-like Growth Factor Binding Protein 4; BP 4; BP4; HT29 IGFBP; IBP 4; IBP4; IGF binding protein 4; IGFBP 4; IBP4_HUMAN.
literatures	Specific References  (1)     |     SL10585R has been referenced in 1 publications. [IF=4.996] Xi, Yue. et al. Low expression of IGFBP4 and TAGLN accelerate the poor overall survival of osteosarcoma. SCI REP-UK. 2022 Jun;12(1):1-20  IF ;  Human, Mouse.   PubMed:35665757
Research Area	Tumour  immunology  Growth factors and hormones  transcriptional regulatory factor  Diabetes
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	26kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IGFBP4: 181-254/254
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Insulin like growth factor binding protein 4 (IGFBP4) is produced from a DNA sequence encoding the human IGFBP4 protein. Mature human IGFBP4 has a calculated molecular mass of approximately 26 kDa. Due to glycosylation, the recombinant protein migrates as a 32 kDa and 25 kDa protein under reducing and non reducing conditions, respectively. Human IGFBP4 has a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both mouse and rat IGFBP4. IGFBP4 is a member of the superfamily of insulin-like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). IGFBP4 functions as an inhibitor of IGF action and its main function may be to protect cells from overstimulation by IGFs or to allow activation of alternate transmembrane signaling pathways that are inhibited by IGF exposure. IGFBP4 is expressed in multiple tissues including adrenal, testis, spleen, heart, lung, kidney, liver, stomach, hypothalamus, and brain cortex. Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Subunit: Binds IGF2 more than IGF1. Subcellular Location: Secreted. Similarity: Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain. SWISS: P22692 Gene ID: 3487 Database links: Entrez Gene: 374271 Chicken Entrez Gene: 282262 Cow Entrez Gene: 3487 Human Entrez Gene: 16010 Mouse Entrez Gene: 360622 Rat Entrez Gene: 443470 Sheep Omim: 146733 Human SwissProt: Q05716 Cow SwissProt: P22692 Human SwissProt: P47879 Mouse SwissProt: P24854 Pig SwissProt: P21744 Rat SwissProt: Q28893 Sheep Unigene: 462998 Human Unigene: 233799 Mouse Unigene: 160666 Rat
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-IGFBP4 (SL10585R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 27 kD Tissue/cell: Rat adrenal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated(SL10585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated(SL10585R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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