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Product Name His Tag Chinese Name His TagTag anti Alias HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag. literatures Specific References (1) | SL10582R has been referenced in 1 publications.[IF=6.208] Diana Morais. et al. Liposomal Delivery of Newly Identified Prophage Lysins in a Pseudomonas aeruginosa Model. INT J MOL SCI. 2022 Jan;23(17):10143 WB ; Escherichia coli.Product Type Tag anti Immunogen Species Rabbit Clonality Polyclonal React Species Recombinant protein Applications WB=1:2000-5000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Form Liquid Concentration 1mg/ml immunogen KLH conjugated HHHHHH peptide Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS:
N/A
Gene ID:
N/A
Product Picture Sample:
His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4 ug
Primary: Anti-His Tag (SL10582R) at 1/2000 ~ 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug
Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug
Primary: Anti-His tag (SL10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD
Sample:
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug
Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug
Primary: Anti-His tag (SL10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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