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Rabbit Anti-His Tag antibody
Rabbit Anti-His Tag antibody
HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag.
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  • NO.:SL10582R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Recombinant protein
    Applications:WB
    concentration:1mg/ml
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Details

Product Name His Tag
Chinese Name His TagTag anti
Alias HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag.  
literatures
Specific References  (1)     |     SL10582R has been referenced in 1 publications.
[IF=6.208] Diana Morais. et al. Liposomal Delivery of Newly Identified Prophage Lysins in a Pseudomonas aeruginosa Model. INT J MOL SCI. 2022 Jan;23(17):10143  WB ;  Escherichia coli.  
Product Type Tag anti 
Immunogen Species Rabbit
Clonality Polyclonal
React Species Recombinant protein
Applications WB=1:2000-5000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated HHHHHH peptide 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

SWISS:
N/A

Gene ID:
N/A

Product Picture
Sample:
His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4 ug
Primary: Anti-His Tag (SL10582R) at 1/2000 ~ 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug
Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug
Primary: Anti-His tag (SL10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD
Sample:
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug
Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug
Primary: Anti-His tag (SL10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Sample:
His-tagged FMDV VP1 protein (type O) at 30 ug
Primary: Anti-His Tag  (SL10582R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 47 kD

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