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Product Name GOT2 Chinese Name 谷草转氨酶2抗体 Alias ASPATA; FABP pm; Got 2; Aspartate aminotransferase; Aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; EC 2.6.1.1; FABP 1; FABPpm; Fatty acid binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; Glutamate oxaloacetate transaminase 2, mitochondrial; Glutamate oxaloacetate transaminase, mitochondrial; Glutamic oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) ; KAT4; KATIV; Kynurenine aminotransferase IV; mAspAT; mitAAT; Mitochondrial aspartate aminotransferase; OTTMUSP00000017748; Plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A; MGC102129; MGC115763; AL022787; AATM_HUMAN. Research Area Cardiovascular Cell biology Apoptosis transcriptional regulatory factor Kinases and Phosphatases Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization cytoplasmic The cell membrane Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GOT2: 81-180/430 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail GOT2 [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
Function:
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Subunit:
Homodimer.
Subcellular Location:
Mitochondrion matrix. Cell membrane. Note=Exposure to alcohol promotes translocation to the cell membrane.
Similarity:
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
SWISS:
P00505
Gene ID:
2806
Database links:Entrez Gene: 2806 Human
Entrez Gene: 14719 Mouse
Omim: 138150 Human
SwissProt: P00505 Human
SwissProt: P05202 Mouse
Unigene: 599470 Human
Unigene: 230169 Mouse
Unigene: 98650 Rat
Product Picture Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-GOT2 (SL10557R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
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