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Product Name CHRNA7 Chinese Name 烟碱型乙酰胆碱受体α7抗体 Alias CHRFAM7A; ACHA7_HUMAN; cholinergic receptor, nicotinic, alpha 7; Neuronal acetylcholine receptor subunit alpha-7; ACHR ALPHA 7; AChR alpha 7 Receptor; Acra7; ALPHA-7NACHR; ALPHA7; ALPHA7 NICOTINIC ACETYLCHOLINE RECEPTOR; Alpha7 nicr; BTX; CHRNA7; CHRNA7-2; NACHR alpha7; NACHRA7; NARAD; Alpha 7 neuronal nicotinic acetylcholine receptor FAM7A hybrid; CHRNA7 (cholinergic receptor nicotinic alpha 7 exons 5 10) and FAM7A (family with sequence similarity 7A exons A E) fusion; CHRNA7; CHRNA7 DR1; CHRNA7 FAM7A fusion; CHRNA7 FAM7A fusion protein; D 10; D10; MGC120482; MGC120483. nicotinic α7; nicotinicα7; nicotinic α 7; nicotinic α-7; nicotinic-α7. literatures Specific References (9) | SL1049R has been referenced in 9 publications.Research Area Cell biology Neurobiology Signal transduction Apoptosis Channel protein The cell membrane受体 Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CHRNA7: 441-502/502 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The Nicotinic Acetylcholine Receptors are members of a superfamily of ligand gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be hetero pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C terminal extracellular region. The Nicotinic Acetylcholine Receptor alpha 7 forms a homo oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.
Function:
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Subunit:
Homopentamer. Interacts with RIC3; which is required for proper folding and assembly.
Subcellular Location:
Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Similarity:
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.
SWISS:
P36544
Gene ID:
1139
Database links:Entrez Gene: 1139 Human
Entrez Gene: 374001 Chicken
Entrez Gene: 11441 Mouse
Omim: 118511 Human
SwissProt: P22770 Chicken
SwissProt: P36544 Human
SwissProt: Q8IUZ4 Human
SwissProt: P49582 Mouse
Unigene: 88 Cow
Unigene: 511772 Human
Unigene: 113464 Mouse
Unigene: 9698 Rat
Product Picture Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 ug
Lane 2: Cerebrum (Mouse) Lysate at 40 ug
Lane 3: Adrenal glands (Rat) Lysate at 40 ug
Lane 4: Adrenal glands (Mouse) Lysate at 40 ug
Lane 5: Placenta (Mouse) Lysate at 40 ug
Lane 6: Lung (Mouse) Lysate at 40 ug
Lane 7: Testis (Rat) Lysate at 40 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Lane 9: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-CHRNA7 (SL1049R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56’50 kD
Observed band size: 48 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- CHRNA7 (SL1049R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Sample:
Lane 1: Human SH-SY5Y cell Lysates
Lane 2: Human U-87 MG cell Lysates
Lane 3: Human U251 cell Lysates
Primary: Anti-CHRNA7 (SL1049R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kDa
Observed band size: 55kDa
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Placenta (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- CHRNA7 (SL1049R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Sample:
Lymph (Mouse) Lysate at 40 ug
Primary: Anti- CHRNA7 (SL1049R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample: Brain(Mouse) lysate at 30ug;
Primary: Anti- CHRNA7 (SL1049R) at 1:300 dilution ;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size: 55 kD
Observed band size: 55kDTissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(SL1049R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (SL1049R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated(SL1049R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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