Product Name	Podoplanin
Chinese Name	平足蛋白/淋巴管endothelial cells蛋白抗体
Alias	PDPN_HUMAN; DPN; GP36; Aggrus; Glycoprotein 36 (Gp36); PA2.26 antigen; T1-alpha; T1A; 29kDa cytosolic podoplanin intracellular domain; PICD; PSEC0003; PSEC0025;
literatures	Specific References  (8)     |     SL1048R has been referenced in 8 publications. [IF=8.213] Yiqi Yanget al. Bioprinting of an osteocyte network for biomimetic mineralization. Biofabrication . 2020 Jul 29;12(4):045013.  ICC ;  mouse.   PubMed:32610301 [IF=5.391] Choy MV et al. Can we enhance osteoporotic metaphyseal fracture healing through enhancing ultrastructural and functional changes of osteocytes in cortical bone with low-magnitude high-frequency vibration?. FASEB J. 2020 Jan 21.  IHC-P ;  Rat.   PubMed:31961009 [IF=4.202] Majumder et al. A practical and sensitive method of quantitating lymphangiogenesis in vivo. (2013) Lab.Inves. 93:779-91  IF(IHC-F) ;  Mouse.   PubMed:23711825 [IF=3.829] Umezawa K et al. Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lunginjury through augmentation of CXC chemokine production from alveolar epithelial cells. Respir Res. 2019 Jan 11;20(1):9.  WB&ICF ;  Mouse.   PubMed:30634975 [IF=3.622] Zhu Xiaoying. et al. LRRC superfamily expression in stromal cells predicts the clinical prognosis and platinum resistance of ovarian cancer. BMC MED GENOMICS. 2023 Dec;16(1):1-16  IHC ;  Human.   PubMed:36653841 [IF=2.751] Ziqian Liu. et al. Eplerenone ameliorates lung fibrosis in unilateral ureteral obstruction rats by inhibiting lymphangiogenesis. EXP THER MED. 2022 Oct;24(4):1-12  IHC ;  Rat.   PubMed:10.3892/etm.2022.11560 [IF=1.487] Yuefeng Gaoet al. Vitamin E promotes ovine Sertoli cell proliferation by regulation of genes associated with cell division and the cell cycle. Anim Biotechnol . 2020 Jul 2;1-9.  WB ;  sheep.   PubMed:32615852 [IF=1.48] Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular Medicine Reports.  other ;   PubMed:25892295
Research Area	Tumour  Cardiovascular  Cell biology  immunology  lymphocyte  endothelial cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	15kDa
Detection molecular weight	36-40 kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse Podoplanin: 91-166/166
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Function: May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. Subcellular Location: Membrane; Single-pass type I membrane protein. Cell projection, filopodium membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Note=Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. Tissue Specificity: Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. Post-translational modifications: Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. The N-terminus is blocked. Similarity: Belongs to the podoplanin family. SWISS: Q62011 Gene ID: 14726 Database links: Entrez Gene: 10630 Human Entrez Gene: 14726 Mouse Entrez Gene: 54320 Rat Omim: 608863 Human SwissProt: Q86YL7 Human SwissProt: Q62011 Mouse SwissProt: Q64294 Rat Unigene: 468675 Human Unigene: 2976 Mouse Unigene: 794 Rat Podoplanin Protein是一种肾小球足状突The cell membrane粘蛋白，目前已成为一种新的淋巴管内皮标记物蛋白，在少数小静脉上也有表达。 podoplanin Protein是淋巴管内皮高度敏感且特异性的标记物，作为Tumour细胞的血小板聚集介导因子而发挥作用，能促进Tumour细胞迁移及增加Tumour细胞侵袭力。
Product Picture	Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- Podoplanin (SL1048R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 37 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Podoplanin) Polyclonal Antibody, Unconjugated (SL1048R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Podoplanin Protein/gp36 Polyclonal Antibody, Unconjugated(SL1048R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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