Product Name	NR5A2
Chinese Name	成纤维The nucleus受体Nr5a2抗体
Alias	NR5A2/LRH1; Alpha-1-fetoprotein transcription factor; B1-binding factor; B1F; B1F2; CPF; CYP7A promoter-binding factor; FTF; FTZ F1; FTZ F1beta; FTZ-F1; FTZ-F1beta; hB1F 2; HB1F; hB1F-2; Hepatocytic transcription factor; Liver receptor homolog 1; LRH-1; LRH1; Nr5a2; NR5A2_HUMAN; Nuclear receptor subfamily 5 group A member 2.
Research Area	Cell biology  Developmental biology  Chromatin and nuclear signals  Microbiology  Signal transduction  Stem cells  transcriptional regulatory factor  Bacteria and viruses  Cell Surface Molecule  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Cow, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	61kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NR5A2/LRH1: 151-250/541
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	NR5A2 is a pre-adipocyte-specific nuclear receptor that regulates expression of aromatase in adipose tissue. NR5A2 belongs to the fushi tarazu factor-1 subfamily of orphan nuclear receptors. NR5A2 transcripts are abundant in the human ovary and testis and are predominantly expressed in tissues of endodermal origin. NR5A2 is a positive transcription factor for ABCG5 and ABCG8 and regulates genes involved in sterol and bile acid secretion from liver and intestine. It induces cell proliferation through the concomitant induction of cyclin D1 and E1, an effect that is potentiated by its interaction with beta-catenin. Function: Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Subcellular Location: Nucleus. Tissue Specificity: Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in hepG2. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and hepG2 cells. Similarity: Belongs to the nuclear hormone receptor family. NR5 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: O00482 Gene ID: 2494 Database links: Entrez Gene: 541305 Cow Entrez Gene: 2494 Human Entrez Gene: 26424 Mouse Entrez Gene: 60349 Rat Omim: 604453 Human SwissProt: O00482 Human SwissProt: P45448 Mouse SwissProt: Q9QWM1 Rat Unigene: 33446 Human Unigene: 16794 Mouse Unigene: 42941 Rat
Product Picture	Sample: Liver(Rat) Lysate at 40 ug Primary: Anti-NR5A2 (SL10444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 61 kD Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NR5A2 Polyclonal Antibody, Unconjugated(SL10444R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR5A2) Polyclonal Antibody, Unconjugated (SL10444R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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