Product Name	NeuN
Chinese Name	神经元核抗原抗体
Alias	RFOX3_HUMAN; RNA binding protein fox-1 homolog 3; RBFOX3; Fox-1 homolog C; Neuronal nuclei antigen (NeuN antigen); FOX3; FOX-3; HRNBP3;
literatures	Specific References  (8)     |     SL10394R has been referenced in 8 publications. [IF=9.038] Zhi Li. et al. Long-term exposure to 2-amino-3-methylimidazo[4,5-f]quinoline can trigger a potential risk of Parkinson's disease. J Hazard Mater. 2021 Jun;412:125230  IF ;  Zebrafish.   PubMed:10.1016/j.jhazmat.2021.125230 [IF=6.291] Hongmei Zhou. et al. Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain. Ecotox Environ Safe. 2022 Jan;229:113066  IF ;  Mouse.   PubMed:34929507 [IF=6.208] Jia Wang. et al. FOXG1 Contributes Adult Hippocampal Neurogenesis in Mice. INT J MOL SCI. 2022 Jan;23(23):14979  IHC, IF ;  Mouse.   PubMed:36499306 [IF=4.831] Haoqiang Zhang. et al. Liraglutide improved the cognitive function of diabetic mice via the receptor of advanced glycation end products down-regulation.. Aging-Us. 2021 Jan 15; 13(1): 525–536  IF ;  Fish.   PubMed:33298623 [IF=4.652] Zhang et al. The Neuroprotective Effect of Puerarin in Acute Spinal Cord Injury Rats. (2016) Cell.Physiol.Bioche. 39:1152-64  IHC-P ;  Rat.   PubMed:27576607 [IF=4.398] Danyang Li. et al. Heterozygous disruption of beclin 1 alleviates neurotoxicity induced by sub-chronic exposure of arsenite in mice. NEUROTOXICOLOGY. 2022 Oct;:  IHC ;  Mouse.   PubMed:36374725 [IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36.  IF ;  Mouse.   PubMed:29678591 [IF=2.784] Xiong LL et al. Tree shrew neural stem cell transplantation promotes functional recovery of tree shrews with a hemi‑sectioned spinal cord injury by upregulating nerve growth factor expression. Int J Mol Med. 2018 Jun;41(6):3267-3277.  IF ;  tree shrews.   PubMed:29532893
Research Area	Cell biology  Neurobiology  Apoptosis  Transporter  Cell type markers  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Horse, Sheep, Bee, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	34kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FOX3: 101-200/312
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017] Function: RNA-binding protein that regulates alternative splicing events. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: A6NFN3 Gene ID: 146713 Database links: Entrez Gene: 146713 Human Entrez Gene: 52897 Mouse Entrez Gene: 287847 Rat SwissProt: A6NFN3 Human SwissProt: Q8BIF2 Mouse Unigene: 135229 Human Unigene: 341103 Mouse Unigene: 143966 Rat
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(SL10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(SL10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NeuN) polyclonal Antibody, Unconjugated (SL10394R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:A549. Primary Antibody (green line): Rabbit Anti-NeuN antibody (SL10394R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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