Product Name	LPA2
Chinese Name	溶血磷脂酸受体蛋白2抗体
Alias	Endothelial Cell Differentiation Gene 4; Endothelial differentiation lysophosphatidic acid G protein coupled receptor 4; IPA2; LPA receptor 2; LPA receptor EDG4; LPA2; LPAR2; Lysophosphatidic acid receptor Edg4; Edg4; LPAR2_HUMAN.
literatures	Specific References  (2)     |     SL10368R has been referenced in 2 publications. [IF=4.384] Xue-min ZHANG. et al. Effects of LPA on the development of sheep in vitro fertilized embryos and attempt to establish sheep embryonic stem cells. J INTEGR AGR. 2022 Aug;:  WB ;  Sheep.   PubMed:10.1016/j.jia.2022.08.111 [IF=3.575] Mizuki Uenaka. et al. LPA2 promotes neuronal differentiation and neurite formation in neocortical development. Biochem Bioph Res Co. 2022 Apr;598:89  IHC ;  Mouse.   PubMed:35151977
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LPA2: 1-100/351 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008] Function: Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation. Subunit: Interacts with SLC9A3R2/NHERF2, MAGI3 and PLCB3. Interacts with RALA and ADRBK1. Subcellular Location: Cell surface. Cell membrane; Multi-pass membrane protein. Note=Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles. Tissue Specificity: Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q9HBW0 Gene ID: 9170 Database links: Entrez Gene: 9170 Human Entrez Gene: 53978 Mouse Omim: 605110 Human SwissProt: Q9HBW0 Human SwissProt: Q9JL06 Mouse Unigene: 122575 Human Unigene: 23253 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LPA2) Polyclonal Antibody, Unconjugated (SL10368R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LPA2 Polyclonal Antibody, Unconjugated(SL10368R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: mouse thymouses(blue) Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green). Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody: Rabbit IgG,Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody: Goat anti-rabbit IgG-FITC,Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody: rabbit Anti-LPA2 (SL10368R),Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-LPA2 antibody (SL10368R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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