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Product Name GLS1 Chinese Name 谷氨酰胺酶1/Glutaminase抗体 Alias mitochondrial; AAD20; GLS; GLS1; GLSK_HUMAN; Glutaminase kidney isoform; K-glutaminase; KIAA0838; L-glutamine amidohydrolase; DKFZp686O15119; FLJ10358. literatures Specific References (1) | SL10341R has been referenced in 1 publications.[IF=2.343] Tsai HW et al. Inhibition of glutaminase to reverse fibrosis in iatrogenic laryngotracheal stenosis. Laryngoscope. 2020 Jan 6. IHC-P ; Human.Research Area Tumour Cell biology Neurobiology Signal transduction Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 72kDa Cellular localization cytoplasmic Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GLS1: 101-200/669 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012].
Function:
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
Subunit:
Heterotetramer. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.
Subcellular Location:
Isoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion.
Tissue Specificity:
Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
Similarity:
Belongs to the glutaminase family.
Contains 2 ANK repeats.
SWISS:
O94925
Gene ID:
2744
Database links:Entrez Gene: 2744 Human
Entrez Gene: 14660 Mouse
Omim: 138280 Human
SwissProt: O94925 Human
SwissProt: D3Z7P3 Mouse
Unigene: 116448 Human
Unigene: 30102 Mouse
Unigene: 5762 Rat
Product Picture Sample:
Lane 1: Human U251 cell lysates
Lane 2: Human HeLa cell lysates
Primary: Anti-GLS1 (SL10341R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 75 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (SL10341R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLS1) Polyclonal Antibody, Unconjugated (SL10341R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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