Product Name	IFITM1
Chinese Name	Interferon诱导Transmembrane protein1抗体
Alias	CD225; CD225 antigen; CD 225; CD-225; IFI17; Interferon induced protein 17; Interferon induced transmembrane protein 1; Interferon inducible protein 9-27; Interferon-induced protein 17; Interferon-induced transmembrane protein 1; Interferon-inducible protein 9-27; Leu 13 antigen; Leu-13 antigen; LEU13; IFM1_HUMAN; Dispanin subfamily A member 2a; DSPA2a.
literatures	Specific References  (1)     |     SL1031R has been referenced in 1 publications. [IF=2.934] Jingying Xieet al. Host antiviral protein IFITM2 restricts pseudorabies virus replication. Virus Res . 2020 Oct 2;287:198105.  WB ;  pig.   PubMed:32745511
Research Area	Tumour  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	11.7kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IFITM1: 58-86/125 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	IFITM1 expression is induced by interferons alpha and gamma and it is thought to play a role in control of cell growth. It is upregulated in several tumor types and may be useful as a tumor biomarker. Function: IFN-induced antiviral protein that mediate cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus, and dengue virus by inhibiting the early step(s) of replication. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activation or by arresting cell growth in G1 phase in a p53-dependent manner. Implicated in the control of cell growth. Component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals. Subunit: Interacts with CAV1; this interaction enhances the ability of CAV1 in inhibiting ERK activation. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus. Similarity: Belongs to the CD225 family. SWISS: P13164 Gene ID: 8519 Database links: Entrez Gene: 8519 Human Entrez Gene: 68713 Mouse Entrez Gene: 293618 Rat Omim: 604456 Human SwissProt: P13164 Human SwissProt: Q9D103 Mouse Unigene: 458414 Human Unigene: 175661 Mouse Unigene: 22087 Rat IFITM1蛋白是蛋白复合物的组分之一,主要功能是参与同型粘附和抗增殖信号的转导,与细胞增殖、Tumour分化、抗病毒、免疫监视等有关。IFITM-1主要用于消化系统Tumour方面的研究，有学者认为：Interferon诱导Transmembrane protein1与Interferon结合，可以抑制Tumour细胞的分化和增值。
Product Picture	Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IFITM1/CD225 Polyclonal Antibody, Unconjugated(SL1031R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Raji (blue). Primary Antibody: Rabbit Anti-IFITM1 antibody(SL1031R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (SL1031R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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