Product Name	Hrk
Chinese Name	BCL2相互作用蛋白质抗体
Alias	Activator of apoptosis harakiri; Activator of apoptosis Hrk; BCL2 interacting protein; BH3 interacting domain protein 3; BID3; DP5; Harakiri BCL2 interacting protein; Human brain 3UTR of mRNA for neuronal death protein partial sequence; Neuronal death protein DP5; HRK_HUMAN.
Research Area	Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	10kDa
Cellular localization	The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Activator of apoptosis harakiri: 1-50/91
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] Function: Promotes apoptosis. Subunit: Interacts with BCL2 and BCL2L1. Interacts with C1QBP. Subcellular Location: Membrane; Single-pass membrane protein. Mitochondrion. SWISS: O00198 Gene ID: 8739 Database links: Entrez Gene: 8739 Human Entrez Gene: 12123 Mouse Omim: 603447 Human SwissProt: O00198 Human SwissProt: P62816 Mouse SwissProt: P70678 Mouse Unigene: 87247 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hrk) Polyclonal Antibody, Unconjugated (SL10302R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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