Product Name	NSE
Chinese Name	神经元特异性烯醇化酶/γ 烯醇化酶抗体
Alias	Gamma-enolase; Gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enolase; NSE; ENOG_HUMAN; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal.
literatures	Specific References  (8)     |     SL1027R has been referenced in 8 publications. [IF=9.075] Li, Jun. et al. Immunoproteasome inhibition prevents progression of castration-resistant prostate cancer. BRIT J CANCER. 2023 Jan;:1-14  IHC,IF ;  Mouse,Human.   PubMed:36681728 [IF=5.479] Zheng X et al. Electrochemiluminescent immunoassay for neuron specific enolase by using amino-modified reduced graphene oxide loaded with N-doped carbon quantum dots. Mikrochim Acta. 2019 Nov 20;186(12):817.  sandwich ECL immunoassay ;  Hylocereus undatus (H. undatus).   PubMed:31749073 [IF=3.44] Huang, Xiaopeng, et al. "A novel reverse fluorescent immunoassay approach for sensing human chorionic gonadotropin based on silver-gold nano-alloy and magnetic nanoparticles." Analytical and bioanalytical chemistry (2015): 1-9.  other ;   PubMed:26547191 [IF=2.94] Zhang, Shuai-nan, et al. "Cerebral potential biomarkers discovery and metabolic pathways analysis of α-synucleinopathies and the dual effects of Acanthopanax senticosus Harms on central nervous system through metabolomics analysis." Journal of ethnopharmacology (2015).  WB ;  Mouse.   PubMed:25660332 [IF=2.234] Lu et al. Autophagy activator promotes neuronal differentiation of adult adipose-derived stromal cells. (2013) Neural.Regen.Res. 8:882-9  ICC ;  Human.   PubMed:25206379 [IF=2.2] Wang, Nan, et al. "Myocardin-related transcription factor-A is key regulator in retinoic acid-induced neural-like differentiation of adult bone marrow-derived mesenchymal stem cells." Gene (2013).  WB ;  Rat.   PubMed:23541806 [IF=1.57] LI, Guang-Zhou, and Feng TIAN. ʺGuanine-Decorated Graphene Nanostructures for Sensitive Monitoring of Neuron-Specific Enolase Based on an Enzyme-Free Electrocatalytic Reaction.ʺ Analytical Sciences 29 (2013): 1195.  other ;   PubMed:24334987 [IF=1.22] Yan et al. Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage. (2017) Iran.J.Basic.Med.Sc. 20:1029-1036  IF(ICC) ;  Rat.   PubMed:29085598
Research Area	Tumour  Cell biology  immunology  Neurobiology  TumourCell biologyMaker
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Dog,  (predicted: Chicken, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test  IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NSE: 201-300/434
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]. Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. Subunit: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Subcellular Location: Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. Tissue Specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. Similarity: Belongs to the enolase family. SWISS: P09104 Gene ID: 2026 Database links: Entrez Gene: 2026 Human Entrez Gene: 13807 Mouse Entrez Gene: 24334 Rat Omim: 131360 Human SwissProt: P09104 Human SwissProt: P17183 Mouse SwissProt: P07323 Rat Unigene: 511915 Human 神经元特异性烯醇化酶（NSE）存在于神经元细胞和神经内分泌组织中，起源于神经内分泌细胞的Tumour可产生过量的NSE。NSE也是小细胞肺癌的检测指标，70％左右的小细胞肺癌患者血中NSE升高，而其他组织型肺癌NSE升高的患者仅为10％～20％。
Product Picture	Sample: Brain (Mouse) Lysate at 30 ug Cerebellum (Mouse) Lysate at 30 ug Primary: Anti-NSE (SL1027R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 49kD Sample: Spinal cord (Mouse) Lysate at 40 ug Spinal cord (Rat) Lysate at 40 ug Primary: Anti-NSE (SL1027R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Sample: A431 Cell Lysate at 40 ug Primary: Anti- NSE (SL1027R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 48 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Polyclonal Antibody, Unconjugated (SL1027R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Polyclonal Antibody, Unconjugated (SL1027R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Polyclonal Antibody, Unconjugated (SL1027R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Polyclonal Antibody, Unconjugated (SL1027R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: Neuroblastoma cells; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NSE/ENO2/γ Enolase Polyclonal Antibody, Unconjugated(SL1027R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: dog bladder tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NSE/ENO2/γ Enolase Polyclonal Antibody, Unconjugated(SL1027R) 1:800, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: U-87MG(blue). Primary Antibody:Rabbit Anti-NSE antibody(SL1027R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (SL1027R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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