Product Name	COX4I1
Chinese Name	细胞色素c氧化酶IV亚型1抗体
Alias	COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX-IV; COX IV; COX 4; COX 4I1;
literatures	Specific References  (7)     |     SL10257R has been referenced in 7 publications. [IF=5.48] Wu, Bin, et al. "Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2017).  WB ;  Mouse.   PubMed:28634116 [IF=4.427] Wang Y et al.Fine particulate matter induces mitochondrial dysfunction and oxidative stress in human SH-SY5Y cells.(2018) Chemosphere;218:577-588.  WB ;   PubMed:30502696 [IF=3.83] Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653  WB ;  Human.   PubMed:28974930 [IF=2.639] Yue Wang # et al. Mitophagy Induced by Mitochondrial Function Damage in Chicken Kidney Exposed to Cr(VI). Biol Trace Elem Res. 2021 Feb;199(2):703-711.  WB ;  Chicken.   PubMed:32440992 [IF=2.197] Zhang JT et al. CRISPR/Cas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFα/CHX-induced Apoptosis. BioMed Research International.2019, Article ID 9071297.  WB ;  Human.   PubMed:doi:10.1155/2019/9071297 [IF=2.1] Wang, Da-Ting, et al. "Artemisinin mimics calorie restriction to trigger mitochondrial biogenesis and compromise telomere shortening in mice." PeerJ 3 (2015): e822.  WB ;  Mouse.   PubMed:25780774 [IF=1.69] Gao, Jinhang, et al. "De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer." Molecular medicine reports 12.5 (2015): 7033-7038.  WB ;  Human.   PubMed:26323487
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human COX4I1: 51-169/169
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Subcellular Location: Mitochondrion inner membrane. Post-translational modifications: Ubiquitous. Similarity: Belongs to the cytochrome c oxidase IV family. SWISS: P13073 Gene ID: 1327 Database links: Entrez Gene: 1327 Human Entrez Gene: 12857 Mouse Entrez Gene: 29445 Rat SwissProt: P13073 Human SwissProt: P19783 Mouse SwissProt: P10888 Rat
Product Picture	Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Heart tissue lysates Lane 3: Mouse Muscle tissue lysates Lane 4: Rat Kidney tissue lysates Lane 5: Rat Heart tissue lysates Lane 6: Rat Muscle tissue lysates Lane 7: Human HeLa cell lysates Lane 8: Human Jurkat cell lysates Lane 9: Human THP-1 cell lysates Lane 10: Human Raji cell lysates Lane 11: Human HepG2 cell lysates Lane 12: Human MCF-7 cell lysates Primary: Anti-COX4I1 (SL10257R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 16 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Polyclonal Antibody, Unconjugated (SL10257R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-COX4 Polyclonal Antibody, Unconjugated(SL10257R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Positive control: Hela cells Concebtration: 5μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice.

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