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Product Name Visfatin Chinese Name 内脂素/内脏脂肪素/前B细胞克隆增强因子1抗体 Alias pre-B-cell colony-enhancing factor 1; PBEF1 protein; AI480535; 1110035O14Rik; AI314458; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre B cell colony enhancing factor 1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; NAMPT_HUMAN. Research Area Cardiovascular immunology Neurobiology Growth factors and hormones Diabetes Endocrinopathy Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Chicken, Dog, Pig, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PBEF: 401-491/491 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011].
Function:
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
Subunit:
Homodimer.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Similarity:
Belongs to the NAPRTase family.
SWISS:
P43490
Gene ID:
10135
Database links:Entrez Gene: 10135 Human
Entrez Gene: 59027 Mouse
Omim: 608764 Human
SwissProt: P43490 Human
SwissProt: Q99KQ4 Mouse
Unigene: 489615 Human
Unigene: 202727 Mouse
Unigene: 203508 Rat
Product Picture Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Visfatin (SL10245R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-HAUS3 antibody (SL15414R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution:0.2μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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