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Product Name HINT1 Chinese Name 组氨酸三聚体核苷Binding protein1抗体 Alias histidine triad nucleotide binding protein 1; Adenosine 5' monophosphoramidase; Adenosine 5'-monophosphoramidase; HINT 1; HINT-1; histidine triad nucleotide binding protein 1; histidine triad nucleotide binding protein; histidine triad nucleotide-binding protein; Histidine triad nucleotide-binding protein 1; PKCI 1; PKCI-1; PKCI1; PRKCNH1; Protein kinase C inhibitor 1; Protein kinase C interacting protein 1; Protein kinase C-interacting protein 1; HINT1_HUMAN. Research Area Tumour Cell biology immunology Binding protein Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 14kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HINT1: 41-126/126 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012].
Function:
Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
Subunit:
Homodimer. Interacts with CDK7.
Subcellular Location:
Cytoplasm. Nucleus. Note=Interaction with CDK7 leads to a more nuclear localization.
Tissue Specificity:
Widely expressed.
Similarity:
Belongs to the HINT family.
Contains 1 HIT domain.
SWISS:
P49773
Gene ID:
3094
Database links:Entrez Gene: 3094 Human
Entrez Gene: 15254 Mouse
Omim: 601314 Human
SwissProt: P49773 Human
SwissProt: P70349 Mouse
Unigene: 483305 Human
Unigene: 425 Mouse
Unigene: 138667 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HINT1) Polyclonal Antibody, Unconjugated (SL10228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HINT1) Polyclonal Antibody, Unconjugated (SL10228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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