Product Name	CMKLR1
Chinese Name	趋化样因子受体1抗体
Alias	Chemokine receptor-like 1; CHEMR23; ChemR23; DEZ; Chemokine like receptor 1; G protein coupled receptor ChemR23; G protein coupled receptor DEZ; CML1_HUMAN ; Orphan G protein coupled receptor.
literatures	Specific References  (4)     |     SL10185R has been referenced in 4 publications. [IF=7.58] Qu, Xinli, et al. "Resolvins E1 and D1 inhibit interstitial fibrosis in the obstructed kidney via inhibition of local fibroblast proliferation." The Journal of pathology 228.4 (2012): 506-519.  WB, IHC-F ;  Mouse.   PubMed:22610993 [IF=5.195] Jing-Xiao Wang. et al. Chemerin-9 in paraventricular nucleus increases sympathetic outflow and blood pressure via glutamate receptor-mediated ROS generation. EUR J PHARMACOL. 2022 Dec;936:175343  WB ;  Rat.   PubMed:36306926 [IF=3.61] Peng, Lei, et al. "The Chemerin Receptor CMKLR1 is a Functional Receptor for Amyloid-β Peptide." Journal of Alzheimers Disease (2014).  Mouse.   PubMed:25079809 [IF=3.448] Hanthazi A et al. Chemerin influences endothelin-and serotonin-induced pulmonary artery vasoconstriction in rats. Life Sci. 2019 Jun 16:116580.  IHC-P ;  Rat.   PubMed:31216440
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  G protein-coupled receptor  Epigenetics  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	42kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from rat CMKLR1: 151-250/371 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Chemokine-Like Receptor 1 (CMKLR1) is a serpentine transmembrane G-protein linked receptor. It is an orphan chemoattractant receptor, also known as ChemR23 or DEZ. CMKLR1 is expressed on the surface of monocyte-derived immature dendritic cells and peripheral plamacytoid dendritic (pDC) cells; but not on myeloid dendritic (mDC) cells, monocytes and lymphocytes. The expression of CMKLR1 on pDC is down-regulated after activation. Chemerin (TIG2) is a natural ligand of CMKLR1. The interaction of CMKLR1 with Chemerin induces pDC activation and chemotaxis. It has been shown that CMKLR1 is a coreceptor for several strains of SIV and HIV-1. CMKLR1 is a potential useful marker for distinguishing pDCs and mDCs. Function: Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 induces activation of intracellular signaling molecules, such as SKY, MAPK1/3 (ERK1/2), MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2). Subcellular Location: Cell membrane. Tissue Specificity: Prominently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands. Prominently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands. Expressed in cardiovascular system, brain, kidney, gastrointestinal tissues and myeloid tissues. Expressed in a broad array of tissues associated with hematopoietic and immune function including, spleen, thymus, appendix, lymph node, bone marrow and fetal liver. Among leukocyte populations abundant expression in monocyte-derived macrophage and immature dendritic cells (DCs). High expression in blood monocytes and low levels in polymorphonuclear cells and T cells. Expressed on endothelial cells. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q99788 Gene ID: 1240 Database links: Entrez Gene: 1240 Human Entrez Gene: 14747 Mouse Entrez Gene: 60669 Rat Omim: 602351 Human SwissProt: Q99788 Human SwissProt: P97468 Mouse SwissProt: O35786 Rat Unigene: 197143 Human Unigene: 5196 Mouse Unigene: 44465 Rat
Product Picture	Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CMKLR1 Polyclonal Antibody, Unconjugated(SL10185R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CMKLR1 Polyclonal Antibody, Unconjugated(SL10185R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Black line : Positive blank control HUVEC); Negative blank control (A431） Green line : Primary Antibody (Rabbit Anti-CMKLR1 antibody (SL10185R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488) HUVEC（Positive）and A431(Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMKLR1 Antibody(SL10185R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

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