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Product Name phospho-CD130/gp130 (Ser782) Chinese Name 磷酸化gp130抗体 Alias gp130 (phospho S782); gp130 (phospho Ser782); p-gp130 (Ser782); Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS; gp130 transducer chain; GP130-RAPS; IL6 ST; IL6R-beta; IL6ST.IL6R-beta; IL6RB_HUMAN; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB. Product Type Phosphorylated anti Research Area Cell biology Signal transduction The cell membrane受体 Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Cellular localization The cell membrane Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human gp130 around the phosphorylation site of Ser782: SE(p-S)TQ Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Function:
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.
Subunit:
Interacts with INPP5D/SHIP1. Forms heterodimers composed of LIPR and IL6ST (type I OSM receptor). Also forms heterodimers composed of OSMR and IL6ST (type II OSM receptor). Homodimer. The homodimer binds two molecules of herpes virus IL6. Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Interacts with HCK.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.
Tissue Specificity:
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Post-translational modifications:
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated.
Similarity:
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
SWISS:
P40189
Gene ID:
3572
Database links:Entrez Gene: 3572 Human
Entrez Gene: 16195 Mouse
Omim: 600694 Human
SwissProt: P40189 Human
SwissProt: Q00560 Mouse
Unigene: 532082 Human
Unigene: 706627 Human
Unigene: 4364 Mouse
Unigene: 12138 Rat
Product Picture Sample:
293T (human)cell Lysate at 40 ug
HepG2 (human)cell Lysate at 40 ug
Primary: Anti- p-gp130 (Ser782) (SL10122R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113kD
Observed band size: 123 kD
Sample: Raji (human)cell Lysate at 40 ug
Primary: Anti- p-gp130 (Ser782) (SL10122R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 113kD
Observed band size: 123 kD
Sample:
Lane 1: Human HepG2 cell Lysates
Lane 2: Human HUVEC cell Lysates
Primary: Anti-phospho-CD130gp130 (Ser782) (SL10122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130kDa
Observed band size: 130kDa
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: A549 (Human) Cell Lysate at 30 ug
Lane 4: U87MG (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-CD130/gp130 (Ser782) (SL10122R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 140 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (SL10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-gp130 (Ser782)) Polyclonal Antibody, Unconjugated (SL10122R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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