Product Name	Runx3
Chinese Name	Runx3抗体
Alias	RUNX3_HUMAN; Runt-related transcription factor 3; AML2; CBFA3; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3 (CBF-alpha-3); Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit (PEA2-alpha C; PEBP2-alpha C); SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit;
literatures	Specific References  (2)     |     SL0378R has been referenced in 2 publications. [IF=3.48] He, Jian?\Feng, et al. "xpression of RUNX3 in salivary adenoid cystic carcinoma: Implications for tumor progression and prognosis." Cancer science 99.7 (2008): 1334-1340..  IHC-P ;  Human.   PubMed:18410404 [IF=2.35] Liu, Yanhua, et al. "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." International Journal of Molecular Medicine (2017).  WB ;  Human.   PubMed:28534977
Research Area	Tumour  Cell biology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Runx3: 151-250/415
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Subunit: Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. Subcellular Location: Nucleus. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Post-translational modifications: Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN. Similarity: Contains 1 Runt domain. SWISS: Q13761 Gene ID: 864 Database links: Entrez Gene: 864 Human Omim: 600210 Human SwissProt: Q13761 Human Unigene: 170019 Human 新型抑癌基因/一种新发现的抑癌基因。经研究发现,在人类胃癌细胞系和胃癌组织中普遍存在Runx3基因表达的缺失或下调,该蛋白与胃癌的发生、发展密切相关。 RUNX3基因被认为是一种新发现的抑癌基因,对胃癌细胞的生长有明显的抑制作用在胃黏膜epithelial cells调控、对脊神经节的神经发育和TCell differentiation中都发挥重要作用。 随着研究的不断深入，Runx3蛋白有望成为胃癌诊断的一个新型生物学Maker和基因治疗靶点。
Product Picture	Sample: A549 Cell Lysate at 30 ug Primary: Anti- Runx3 (SL0378R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD Sample: Lymph node (Mouse) Lysate at 30 ug Primary: Anti- Runx3 (SL0378R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated(SL0378R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Runx3 Antibody(SL0378R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange). Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-Runx3 antibody (SL0378R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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