Product Name	NALP3/CIAS1
Chinese Name	Apoptosis诱导蛋白NALP3抗体
Alias	LRR and PYD domains-containing protein 3; AGTAVPRL; AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like; Angiotensin/vasopressin receptor AII/AVP-like; C1orf7; Caterpiller protein 1.1; CIAS 1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Muckle-Wells syndrome; MWS; NACHT; NACHT LRR and PYD containing protein 3; NALP 3; NALP3; NALP3_HUMAN; NLRP3; PYPAF 1; PYPAF1 antibody PYRIN containing APAF1 like protein 1; PYRIN-containing APAF1-like protein 1.
literatures	Specific References  (66)     |     SL10021R has been referenced in 66 publications. [IF=10.383] Zhen Xu. et al. Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXX  WB ;  Mouse,Human.   PubMed:36881383 [IF=8.469] Pang, Qi. et al. Irisin protects against vascular calcification by activating autophagy and inhibiting NLRP3-mediated vascular smooth muscle cell pyroptosis in chronic kidney disease. Cell Death Dis. 2022 Mar;13(3):1-15  IF ;  Mouse.   PubMed:35354793 [IF=8.469] Zheng, Jie. et al. A novel function of NLRP3 independent of inflammasome as a key transcription factor of IL-33 in epithelial cells of atopic dermatitis. Cell Death Dis. 2021 Sep;12(10):1-12  IHC ;  mouse.   PubMed:34561424 [IF=7.919] Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240.  WB、IHC ;  Mouse.   PubMed:33377656 [IF=7.561] You Zhou. et al. Overexpression of lncRNA TUG1 Alleviates NLRP3 Inflammasome-Mediated Cardiomyocyte Pyroptosis Through Targeting the miR-186-5p/XIAP Axis in Coronary Microembolization-Induced Myocardial Damage. Front Immunol. 2021; 12: 637598  WB ;  Rat.   PubMed:34163467 [IF=7.376] Meng Sun. et al. Silibinin protects against sepsis and septic myocardial injury in an NR1H3-dependent pathway. FREE RADICAL BIO MED. 2022 Jul;187:141  WB ;  Mouse.   PubMed:35640818 [IF=7.129] Jingjing Zhang. et al. The inhibition of TRPML1/TFEB leads to lysosomal biogenesis disorder, contributes to developmental fluoride neurotoxicity. ECOTOX ENVIRON SAFE. 2023 Jan;250:114511  WB ;  Rat,Human.   PubMed:36608573 [IF=6.6] Chinmaya Panda. et al. Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells. Cells-Basel. 2021 Jul;10(7):1652  ICC ;  Human.   PubMed:34209408 [IF=6.6] Maryam Baazm. et al. Regulation of Inflammasomes by Application of Omega-3 Polyunsaturated Fatty Acids in a Spinal Cord Injury Model. Cells-Basel. 2021 Nov;10(11):3147  WB ;  Rat.   PubMed:34831370 [IF=6.543] Wen Lianghe. et al. Melatonin Exerts Cardioprotective Effects by Inhibiting NLRP3 Inflammasome-Induced Pyroptosis in Mice following Myocardial Infarction. Oxid Med Cell Longev. 2021;2021:5387799  WB,IF ;  mouse.   PubMed:34512865 [IF=6.388] Yuanyuan Chen. et al. Inhibiting mitochondrial inflammation through Drp1/HK1/NLRP3 pathway: A mechanism of alpinetin attenuated aging-associated cognitive impairment. PHYTOTHER RES. 2023 Feb;:  WB,IF ;  Mouse.   PubMed:36772986 [IF=6.376] Wang, Xiaoying. et al. LncRNA FENDRR with m6A RNA methylation regulates hypoxia-induced pulmonary artery endothelial cell pyroptosis by mediating DRP1 DNA methylation. MOL MED. 2022 Dec;28(1):1-20  WB, IF ;  Human.   PubMed:36284300 [IF=6.304] Hanwen Li et al. P2Y 14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death Dis.2020 May 26;11(5):394.  WB ;  Rat.   PubMed:32457291 [IF=6.208] Nina Müller. et al. Lipocalin-2 Deficiency Diminishes Canonical NLRP3 Inflammasome Formation and IL-1β Production in the Subacute Phase of Spinal Cord Injury. INT J MOL SCI. 2023 Jan;24(10):8689  WB,IF ;  Mouse.   PubMed:37240031 [IF=6.187] Debye et al. Neurodegeneration and NLRP3 inflammasome expression in the anterior thalamus of SOD1(G93A) ALS mice. (2016) Brain.Pathol.  IHC-P,IHC-P-IF ;  Mouse.   PubMed:27880990 [IF=5.895] Xu Yang. et al. T-2 Toxin Caused Mice Testicular Inflammation Injury via ROS-Mediated NLRP3 Inflammasome Activation. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX  WB ;  Mouse.   PubMed:36260425 [IF=5.714] Lu Zhan. et al. Honokiol reduces fungal burden and ameliorate inflammation lesions of Aspergillus fumigatus keratitis via Dectin-2 down-regulation. INT IMMUNOPHARMACOL. 2023 May;118:109849  WB ;  Mouse.   PubMed:36933490 [IF=5.714] Yani He. et al. Glycolytic reprogramming controls periodontitis-associated macrophage pyroptosis via AMPK/SIRT1/NF-κB signaling pathway. INT IMMUNOPHARMACOL. 2023 Jun;119:110192  WB ;  Human.   PubMed:37068341 [IF=5.589]   WB + ELISA ;  Mouse.   PubMed:31863983 [IF=5.4] Zendedel, Adib, et al. "Estrogen Attenuates Local Inflammasome Expression and Activation after Spinal Cord Injury." Molecular Neurobiology (2017): 1-12.  WB, IF(IHC-P) ;  Rat.   PubMed:28127698 [IF=5.4] Heitzer, Marius, et al. "Administration of 17β-Estradiol Improves Motoneuron Survival and Down-regulates Inflammasome Activation in Male SOD1 (G93A) ALS Mice." Molecular Neurobiology: 1-15.  WB ;  Mouse.   PubMed:27957680 [IF=5.396] Shi-Yong Zhu. et al. Lycopene Ameliorates Atrazine-Induced Pyroptosis in Spleen via Suppressing the Ox-mtDNA/Nlrp3 Inflammasome Pathway. Food Funct. 2021 Nov;:  IF ;  Mouse.   PubMed:10.1039/D1FO02857J [IF=5.29] Zendedel, Adib, et al. "Activation and Regulation of NLRP3 Inflammasome by Intrathecal Application of SDF-1a in a Spinal Cord Injury Model." Molecular Neurobiology (2015): 1-13.  WB ;  Rat.   PubMed:25972240 [IF=5.195] Ming-Hui Li. et al. Fufang Zhenzhu Tiaozhi capsule ameliorates hyperuricemic nephropathy by inhibition of PI3K/AKT/NF-κB pathway. J ETHNOPHARMACOL. 2022 Aug;:115644  WB ;  Mouse.   PubMed:35987412 [IF=5.195] Yangchun Liu. et al. Nicorandil protects against coronary microembolization-induced myocardial injury by suppressing cardiomyocyte pyroptosis via the AMPK/TXNIP/NLRP3 signaling pathway. EUR J PHARMACOL. 2022 Dec;936:175365  IF, WB ;  Rat.   PubMed:36336011 [IF=5.195] Zhen Xu. et al. The aqueous extracts of Ageratum conyzoides inhibit inflammation by suppressing NLRP3 inflammasome activation. J ETHNOPHARMACOL. 2023 Jun;309:116353  WB,ICC ;  Mouse,Human.   PubMed:36907476 [IF=5.195] Qian Zhang. et al. Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide-induced acute lung injury. J ETHNOPHARMACOL. 2023 Sep;313:116615  WB ;  Rat，Human.   PubMed:37164255 [IF=5.168] Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.  WB ;  Dog.   PubMed:28465478 [IF=5.1] Zhang, Xiaohui, et al. "H3 Relaxin Protects Against Myocardial Injury in Experimental Diabetic Cardiomyopathy by Inhibiting Myocardial Apoptosis, Fibrosis and Inflammation." Cellular Physiology and Biochemistry 43.4 (2017): 1311-1324.  WB ;  Rat.   PubMed:28992627 [IF=4.966] Hui Che. et al. Melatonin exerts neuroprotective effects by inhibiting neuronal pyroptosis and autophagy in STZ‐induced diabetic mice. Faseb J. 2020 Oct;34(10):14042-14054  WB,IHC ;  Mouse, Human.   PubMed:32910484
Research Area	Tumour  Cardiovascular  Cell biology  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Dog,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	114kDa
Cellular localization	The nucleus cytoplasmic Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Cryopyrin: 15-120/1036
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Function: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. DISEASE: Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Similarity: Belongs to the NLRP family. Contains 1 DAPIN domain. Contains 9 LRR (leucine-rich) repeats. Contains 1 NACHT domain. SWISS: Q96P20 Gene ID: 114548 Database links: Entrez Gene: 114548 Human Entrez Gene: 216799 Mouse Entrez Gene: 287362 Rat Omim: 606416 Human SwissProt: Q96P20 Human SwissProt: Q8R4B8 Mouse Unigene: 159483 Human Unigene: 54174 Mouse
Product Picture	Sample: Raji Cell (Human) Lysate at 30 ug MDA-MB-231 Cell (Human) Lysate at 30 ug Primary: Anti- CIAS1 (SL10021R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114kD Observed band size: 114kD Sample: Thymus (Rat) Lysate at 40 ug Primary: Anti-NALP3’CIAS1 (SL10021R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 120 kD Sample: Spleen (Mouse) Lysate at 40 ug Thymus (Mouse) Lysate at 40 ug Primary: Anti- NALP3’CIAS1 (SL10021R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD Sample: Lane 1: RAW264.7 (Mouse) Cell Lysate at 30 ug Lane 2: Thymus (Mouse) Lysate at 40 ug Primary: Anti- NALP3’CIAS1 (SL10021R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Large intestine (Mouse) Lysate at 40 ug Lane 3: Thymus (Mouse) Lysate at 40 ug Lane 4: Lung (Mouse) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Thymus (Rat) Lysate at 40 ug Lane 7: Lung (Rat) Lysate at 40 ug Lane 8: Jurkat (Human) cell Lysate at 30 ug Primary: Anti-NALP3/CIAS1 (SL10021R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed band size: 118 kD Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti- NALP3’CIAS1 (SL10021R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114 kD Observed band size: 114 kD Sample: Raji (human)cell Lysate at 40 ug MCF-7 (human)cell Lysate at 40 ug siha (human)cell Lysate at 40 ug Primary: Anti- NALP3'CIAS1 (SL10021R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 114kD Observed band size: 114 kD Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated(SL10021R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NALP3/CIAS1) Polyclonal Antibody, Unconjugated (SL10021R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cryopyrin/CIAS1/NALP3 Polyclonal Antibody, Unconjugated(SL10021R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining This image was generously provided by Adib Zendedel, PhD from RWTH Aachen University. Formalin-fixed and paraffin embedded rat spinal cord tissue labeled with Rabbit Anti-Cryopyrin Polyclonal Antibody, Unconjugated (SL10021R) at 1:300 followed by conjugation to a secondary antibody Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NALP3/CIAS1) Polyclonal Antibody, Unconjugated (SL10021R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-NALP3/CIAS1 antibody (SL10021R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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