Product Name	FLAG Tag
Chinese Name	FLAG Tag标签（N端）抗体
Alias	DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag; DDDDK tag (N-T).
literatures	Specific References  (4)     |     SL0965R has been referenced in 4 publications. [IF=4.096] Liyan Wang. et al. Tracing Key Molecular Regulators of Lipid Biosynthesis in Tuber Development of Cyperus esculentus Using Transcriptomics and Lipidomics Profiling. Genes-Basel. 2021 Oct;12(10):1492  WB ;  Cyperus esculentus.   PubMed:34680888 [IF=3.688] Jiangbo Qu. et al. Functional differentiation of two lhx8 paralogs and possible regulatory role of lhx8a in Japanese flounder (Paralichthys olivaceus). Gene. 2022 Apr;817:146201  WB,IP ;  Human.   PubMed:35063574 [IF=3.046] Ghoorun RA et al. Prognostic Significance of FKBP14 in Gastric Cancer. Onco Targets Ther. 2019 Dec 30;12:11567-11577.  WB ;   PubMed:31920344 [IF=2.741] Zhao, Mengmeng. et al. TRIM4-mediated ubiquitination of NSP2 restricts porcine reproductive and respiratory syndrome virus proliferation. BMC VET RES. 2022 Dec;18(1):1-7  IP ;  Human.   PubMed:35637527
Product Type	Tag anti
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Recombinant protein
Applications	WB=1:1000-2000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	MAP of Flag Tag peptide C-terminus
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This is a useful tool for the localisation and characterisation of DYKDDDDK tagged proteins. SWISS: N/A Gene ID: N/A 蛋白质纯化的新途径─应用抗DYKDDDDK标签8肽抗体检测DYKDDDDK标签N端8肽融合蛋白 1） DYKDDDDK标签8肽与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。 2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） DYKDDDDK标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物） 4） DYKDDDDK8肽标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能，不会影响蛋白分泌 5） DYKDDDDK8肽标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。
Product Picture	Sample: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-FLAG Tag (SL0965R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD

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