Product Name	MTNR1B
Chinese Name	褪黑素受体1B抗体
Alias	MT2; melatonin receptor type 1B; melatonin receptor; MEL1B; MEL-1B-R; Mtnr1b; MGC129286;MGC129287; MTNR-1B; Melatonin receptor 1B; MTR1B_HUMAN.
literatures	Specific References  (15)     |     SL0963R has been referenced in 15 publications. [IF=13.007] Shanshan Yu. et al. Melatonin prevents experimental central serous chorioretinopathy in rats. J PINEAL RES. 2022 Apr 29  WB,IHC ;  Rat.   PubMed:35436360 [IF=10.39] Jang, Hoon, et al. "Synergistic effect of melatonin and ghrelin in preventing cisplatin‐induced ovarian damage via regulation of FOXO3a phosphorylation and binding to the p27Kip1 promoter in primordial follicles." Journal of Pineal Research (2017).  IHC-P ;  Mouse.   PubMed:28658519 [IF=5.682] Gao Xinran. et al. Role of Bmal1 in Type 2 Diabetes Mellitus-Related Glycolipid Metabolic Disorder and Neuropsychiatric Injury: Involved in the Regulation of Synaptic Plasticity and Circadian Rhythms. MOL NEUROBIOL. 2023 May;:1-23  WB ;  Mouse.   PubMed:37126129 [IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551  WB,IF,IHC ;  Mouse.   PubMed:32996162 [IF=5.168] Jie Yang. et al. Exposure to Benzo(a)pyrene damages mitochondrial function via suppressing mitochondrial melatonin receptors in ovarian corpus luteum during early pregnancy. CHEM-BIOL INTERACT. 2022 Aug;:110085  WB, IF ;  Rat, Human.   PubMed:35940284 [IF=2.923] Hongwei Duan. et al. Melatonin regulates dihydrotestosterone formation via its membrane receptor in the epididymal epithelial cells of sheep. THERIOGENOLOGY. 2022 Dec;:  WB ;  Sheep.   PubMed:36623430 [IF=2.923] Junyuan Ma. et al. Effects of melatonin on development and hormone secretion of sheep theca cells in vitro. THERIOGENOLOGY. 2023 Mar;198:172  IHC ;  Sheep.   PubMed:36592515 [IF=2.923] Taojie Zhang. et al. MEL regulates miR-21 and let-7b through the STAT3 cascade in the follicular granulosa cells of Tibetan sheep. THERIOGENOLOGY. 2023 Apr;:  WB,IF ;  Sheep.   PubMed:37120893 [IF=2.478] Tabecka-Lonczynska A et al. Melatonin receptors subtypes (MT1 and MT2) in the uterus masculinus of mature male european bison. Biological and seasonal reproductive role.J Physiol Pharmacol. 2018 Feb;69(1):67-73.  WB ;  European bisons.   PubMed:29769422 [IF=2.17] Brockus, K. E., et al. "Dietary melatonin alters uterine artery hemodynamics in pregnant Holstein heifers." Domestic Animal Endocrinology (2015).  Bovine.   PubMed:26641925 [IF=2.08] Wang, S-J., et al. "Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)." Theriogenology 78.7 (2012): 1517-1526.  WB ;  Bovine.   PubMed:22980085 [IF=1.858] Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 Aug;:  IF, IHC ;  Sheep.   PubMed:36018566 [IF=1.778] Zhao Shu-Qin. et al. The in vitro effects of melatonin and Cry gene on the secretion of estradiol from camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jan;74:106497  WB,IF ;  Camel.   PubMed:32799039 [IF=1.778] S.-Q. Zhao. et al. cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jul;76:106609  IHC ;  Camel.   PubMed:33636446 [IF=1.291] Gao Y et al. Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells.Cell Mol Biol Lett. 2019 Mar 12;24:21.  IHC-P,IF&WB ;  Mouse.   PubMed:30915128
Research Area	Cell biology  immunology  Neurobiology  Growth factors and hormones  The cell membrane受体
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MTNR1B: 201-300/362 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. Function: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Expressed in retina and less in brain and hippocampus. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P49286 Gene ID: 4544 Database links: Entrez Gene: 4544 Human Omim: 600804 Human SwissProt: P49286 Human Unigene: 569039 Human 有学者认为：MTNR1B是组成控制褪黑激素信号通路的一部分。 褪黑激素调解人体的昼夜节律，是通过白天和黑夜来控制睡眠和饮食的内部时钟。MTNR1B的进一步研究为高血糖和Diabetes与昼夜节律遭到破坏有直接关系这一观点提供了证据。
Product Picture	Sample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti- MTNR1B (SL0963R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 40 kD Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Cerebellum (Mouse) Lysate at 40 ug Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Lane 5: Cerebellum (Rat) Lysate at 40 ug Lane 6: Jurkat (Human) Cell Lysate at 30 ug Lane 7: Hela (Human) Cell Lysate at 30 ug Lane 8: U87MG (Human) Cell Lysate at 30 ug Primary: Anti-MTNR1B (SL0963R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40/50-55 kD Observed band size: 60/50 kD Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (SL0963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (SL0963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(SL0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(SL0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (MTNR1B) polyclonal Antibody, Unconjugated (SL0963R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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