Product Name	WDR26
Chinese Name	心肌缺血预处理正调节蛋白2抗体
Alias	myocardial ischemic preconditioning upregulated protein 2; WDR26; PRO0852; CDW2; MIP2; CUL4 and DDB1 associated WDR protein 2; Myocardial ischemic preconditioning up regulated protein 2; myocardial ischemic preconditioning upregulated protein 2; WD repeat domain 26; WD repeat-containing protein 26; FLJ21016; WDR26_HUMAN.
literatures	Specific References  (1)     |     SL0932R has been referenced in 1 publications. [IF=10.02] Su, Xiaomin, et al. "LRRC19 expressed in the kidney induces TRAF2/6-mediated signals to prevent infection by uropathogenic bacteria." Nature Communications 5 (2014).  IHC-F ;  Mouse.   PubMed:25026888
Research Area	Cardiovascular  Cell biology  Signal transduction  Cyclin  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	72kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human WDR26: 101-200/514
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. Function: May be involved in MAPK pathways. Subunit: Interacts with DDB1-CUL4A/B E3 ligase complexes. Subcellular Location: Cytoplasm. Tissue Specificity: Broadly expressed, with highest levels in heart and skeletal muscle. Similarity: Contains 1 CTLH domain. Contains 1 LisH domain. Contains 6 WD repeats. SWISS: Q9H7D7 Gene ID: 80232 Database links: Entrez Gene: 80232 Human Entrez Gene: 226757 Mouse Entrez Gene: 498301 Rat SwissProt: Q9H7D7 Human SwissProt: Q8C6G8 Mouse Unigene: 497873 Human Unigene: 289082 Mouse Unigene: 23078 Rat WDR26可能具有通过MAPK信号途径调节细胞增殖的功能。
Product Picture	Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-WDR26 (SL0932R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 70 kD Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(SL0932R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated(SL0932R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: MCF7; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (WDR26) Polyclonal Antibody, Unconjugated (SL0932R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green). Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-WDR26 antibody (SL0932R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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