Product Name	FSH receptor
Chinese Name	促卵泡刺激素受体抗体
Alias	Follicle-stimulating hormone receptor precursor; Follicle stimulating hormone receptor; Follicle stimulating hormone receptor isoform 1; Follitropin receptor; FSHRO; LGR1; ODG1; Follicle-stimulating hormone receptor; FSH-R; FSH R; Fshr; FSHR_HUMAN; MGC141667; MGC141668; ovarian dysgenesis 1.
literatures	Specific References  (18)     |     SL0895R has been referenced in 18 publications. [IF=12.081] Yu-Fang Liu. et al. Effect of melatonin on ATG2B-mediated autophagy regulation in sheep granulosa cells with different FecB genotypes. J PINEAL RES. 2023 May;:e12890  ICC ;  Sheep.   PubMed:37226314 [IF=7.666] Yufang Liu. et al. Effect of Upregulation of Transcription Factor TFDP1 Binding Promoter Activity Due to RBP4 g.36491960G>C Mutation on the Proliferation of Goat Granulosa Cells. CELLS-BASEL. 2022 Jan;11(14):2148  IF ;  Goat.   PubMed:35883591 [IF=5.923] Patrycja Witek. et al. Long-Term Changes in Ovarian Follicles of Gilts Exposed Neonatally to Methoxychlor: Effects on Oocyte-Derived Factors, Anti-Müllerian Hormone, Follicle-Stimulating Hormone, and Cognate Receptors. Int J Mol Sci. 2022 Jan;23(5):2780  WB,IHC ;  Gilts.   PubMed:35269923 [IF=4.996] Wang, Yan. et al. Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum. SCI REP-UK. 2023 Feb;13(1):1-13  IHC ;  Bovine.   PubMed:36808140 [IF=4.85] Xiaohong Han. et al. LncRNA MEG3 regulates ASK1/JNK axis-mediated apoptosis and autophagy via sponging miR-23a in granulosa cells of yak tertiary follicles. CELL SIGNAL. 2023 Apr;:110680  IF,IHC ;  Bovine.   PubMed:37086956 [IF=4.546] Boyang Wan. et al. The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts. Toxins. 2021 Sep;13(9):626  WB,IF ;  weaned gilts.   PubMed:34564630 [IF=4.369] Qing-yan Zhang. et al. Study of differential proteomics in granulosa cells of premature ovarian insufficiency (POI) and the roles and mechanism of RAC1 in granulosa cells. MOL CELL ENDOCRINOL. 2022 Sep;555:111719  FC ;  Human.   PubMed:35850487 [IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).  IHC-P ;  Mouse.   PubMed:29202267 [IF=4.141] Taotao Ji. et al. Effects of N-Acetylcysteine on the Proliferation, Hormone Secretion Level, and Gene Expression Profiles of Goat Ovarian Granulosa Cells. GENES-BASEL. 2022 Dec;13(12):2306  IF ;  Goat.   PubMed:36553574 [IF=4.102] Huaming Xi. et al. FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells. Mol Cell Endocrinol. 2021 Nov;:111505  WB ;  Goat.   PubMed:34774699 [IF=3.188] Zeqi Tang. et al. Seasonal changes in the expression of PACAP, VPAC1, VPAC2, PAC1 and testicular activity in the testis of the muskrat (Ondatra zibethicus). EUR J HISTOCHEM. 2022 Mar 24; 66(2): 3398  IHC ;  Rat.   PubMed:35502591 [IF=2.899] Deng Y et al. Comparison of growth characteristics of cultured granulosa cells from geese follicles at different developmental stages. Biosci Rep. 2018 Apr 27;38(2).  IF ;  Geese.   PubMed:29545316 [IF=2.68] Luo, Qianqian, et al. "Role of SDF-1/CXCR4 and cytokines in the development of ovary injury in chemotherapy drug induced premature ovarian failure mice." Life Sciences (2017).  IHC-P ;  Mouse.   PubMed:28478265 [IF=2.105] Wang J et al. Seasonal expressions of luteinising hormone receptor, follicle-stimulating hormone receptor and prolactin receptor in the epididymis of the male wild ground squirrel (Spermophilus dauricus). (2018) Reprod. Fertil. Dev.  WB&IHC-P ;  squirrel.   PubMed:30509341 [IF=1.858] Shuang Sun. et al. Role of aquaporin 3 in reproductive performance of dairy goats after repeated estrus synchronization stimulation. REPROD DOMEST ANIM. 2023 Apr;:  IF ;  Goat.   PubMed:37026537 [IF=1.778] Zhao Shu-Qin. et al. The in vitro effects of melatonin and Cry gene on the secretion of estradiol from camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jan;74:106497  ICC ;  Camel.   PubMed:32799039 [IF=1.778] S.-Q. Zhao. et al. cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jul;76:106609  IHC ;  Camel.   PubMed:33636446 [IF=1.41] Hu et al. Lentivirus vector-mediated mitofusin-2 overexpression in rat ovary changes endocrine function and promotes follicular development in vivo. (2014) Exp.Ther.Med. 8:731-736  WB ;  Rat.   PubMed:25120590
Research Area	Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	78kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FSHR: 201-300/695 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] Function: Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Subunit: Interacts with ARRB2. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Sertoli cells and ovarian granulosa cells. DISEASE: Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1) [MIM:233300]; also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS) [MIM:608115]. OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis. Similarity: Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. Contains 9 LRR (leucine-rich) repeats. Contains 1 LRRNT domain. SWISS: P23945 Gene ID: 2492 Database links: Entrez Gene: 281172 Cow Entrez Gene: 100861291 Goat Entrez Gene: 2492 Human Entrez Gene: 14309 Mouse Entrez Gene: 397679 Pig Entrez Gene: 25449 Rat Entrez Gene: 443299 Sheep Omim: 136435 Human SwissProt: P35376 Cow SwissProt: P23945 Human SwissProt: P35378 Mouse SwissProt: P49059 Pig SwissProt: P20395 Rat SwissProt: P35379 Sheep Unigene: 1428 Human Unigene: 57155 Mouse Unigene: 162843 Rat FSHR属于G protein-coupled receptor超家族中的glycoprotein亚家族成员，Transmembrane protein。人类的FSHR是由695个氨基酸组成的一条肽链。主要分布于睾丸支持细胞和卵巢颗粒细胞，成熟的FSHR蛋白分子质量为：75kDa。
Product Picture	Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-FSH receptor (SL0895R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 78 kD Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FSH receptor Polyclonal Antibody, Unconjugated(SL0895R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSH receptor) Polyclonal Antibody, Unconjugated (SL0895R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control: HUVEC cells(blue). Primary Antibody:Rabbit Anti-FSH receptor antibody(SL0895R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (SL0895R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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