Product Name	FLAG Tag (CT)
Chinese Name	FLAG Tag标签（C端）抗体
Alias	DDDDK epitope tag; DDDK; ddk; DYKDDDDK; DYKDDDDK epitope tag; DYKDDDDK tag; DYKDDDDK-Tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG-tag.
literatures	Specific References  (4)     |     SL0879R has been referenced in 4 publications. [IF=5.714] Mengmeng Zhao. et al. Interferon inducible porcine 2′, 5′-oligoadenylate synthetase like-1 protein limits porcine reproductive and respiratory syndrome virus 2 infection via the MDA5-mediated interferon-signaling pathway. INT IMMUNOPHARMACOL. 2022 Oct;111:109151  IP, WB ;  Monkey, Pig.   PubMed:36007390 [IF=5.52] Zhang, Zhenfeng, et al. "Human Bocavirus NP1 Inhibits IFN-?? Production by Blocking Association of IFN Regulatory Factor 3 with IFNB Promoter."The Journal of Immunology 189.3 (2012): 1144-1153.   PubMed:22745372 [IF=3.636] Mengmeng Zhao. et al. Molecular cloning and functional analysis of Macaca mulatta STING. Dev Comp Immunol. 2022 Feb;127:104296  WB ;  Monkey.   PubMed:34656644 [IF=3.412] Mengmeng Zhao. et al. Sequence Analysis of Macaca mulatta TRIM4 and Its Role in the Interferon Pathway. Front Vet Sci. 2022; 9: 805301  WB ;  Monkey.   PubMed:35242838
Product Type	Tag anti
Research Area	Chromatin and nuclear signals
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Recombinant protein
Applications	WB=1:5000-20000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Flag Tag peptide
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This is a useful tool for the localisation and characterisation of DDDDK tagged proteins. SWISS: N/A Gene ID: N/A 蛋白质纯化的新途径─应用抗DYKDDDDK标签8肽抗体检测DYKDDDDK标签N端8肽融合蛋白 1） DYKDDDDK标签8肽与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变性的条件下可进行进一步纯化。 2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） DYKDDDDK标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物） 4） DYKDDDDK8肽标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能，不会影响蛋白分泌 5） DYKDDDDK8肽标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。
Product Picture	Sample: Lane1:DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-FLAG Tag (SL0879R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD Sample: Lane 1: DDDDK-Tagged Fusion Protein Overexpression E.coli Lysate (SL41230P) at 8ug Primary: Anti-FLAG Tag (CT) (SL0879R) at 1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa

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