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Product Name Glutathione Reductase Chinese Name 谷胱甘肽还原酶抗体 Alias glutathione reductase; GLUR; Glutathione reductase mitochondrial; GR; Gr1; GRase; GRD 1; GRD1; GSR; MGC78522; GSHR_HUMAN. literatures Specific References (2) | SL0837R has been referenced in 2 publications.[IF=3.69] Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873 WB ; Human.[IF=2.134] Khan et al. Alantolactone induces apoptosis in HepG2 cells through GSH depletion, inhibition of STAT3 activation, and mitochondrial dysfunction. (2013) Biomed.Res.In. 2013:719858 WB ; Human.Research Area immunology Kinases and Phosphatases Mitochondrion Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization Secretory protein Mitochondrion Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Glutathione Reductase: 421-522/522 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Glutathione reductase (GR) is a member of pyridine nucleotide- disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.
Function:
Maintains high levels of reduced glutathione in the cytosol.
Subunit:
Homodimer; disulfide-linked.
Subcellular Location:
Isoform Mitochondrial: Mitochondrion.
Isoform Cytoplasmic: Cytoplasm.
Similarity:
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
SWISS:
P00390
Gene ID:
2936
Database links:Entrez Gene: 2936 Human
Entrez Gene: 14782 Mouse
Omim: 138300 Human
SwissProt: P00390 Human
SwissProt: P47791 Mouse
Unigene: 271510 Human
Unigene: 283573 Mouse
Unigene: 19721 Rat
胱甘肽还原酶为一种黄素酶,可催化氧化型谷胱甘肽转变成还原型谷胱甘肽(GSH),从而提高细胞液中GSH的含量。
GSH具有抵抗自由基氧化对机体损伤的作用,GSH的重要生理功能在于能还原体内生成的过氧化氢(H2O2)。GSH的储量与创伤、Tumour等的预后,以及人体衰老有关。Product Picture Sample:
A549(Human) Cell Lysate at 30 ug
SP2/0(Mouse) Cell Lysate at 30 ug
Primary: Anti-Glutathione Reductase (SL0837R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (SL0837R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human Pulp); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glutathione Reductase) Polyclonal Antibody, Unconjugated (SL0837R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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