Product Name	Oct4
Chinese Name	胚胎Stem cells关键蛋白抗体
Alias	MGC22487; Oct 3; Oct 4; Oct-3; Oct-4; OCT3; Oct4; Octamer binding protein 3; Octamer-binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding transcription factor 3; Octamer-4; POU domain transcription factor OCT4; OTF3; OTF4; OTF 3; OTF 4; OTF-3; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU domain class 5 transcription factor 1; PO5F1_HUMAN; POU type homeodomain containing DNA binding protein ; POU5F1.
literatures	Specific References  (14)     |     SL0830R has been referenced in 14 publications. [IF=8.755] Sijie Wang. et al. PFKFB4 facilitates palbociclib resistance in oestrogen receptor-positive breast cancer by enhancing stemness. CELL PROLIFERAT. 2022 Sep;:e13337  WB ;  Human.   PubMed:36127291 [IF=8.469] Que, Tianshi. et al. HMGA1 stimulates MYH9-dependent ubiquitination of GSK-3β via PI3K/Akt/c-Jun signaling to promote malignant progression and chemoresistance in gliomas. Cell Death Dis. 2021 Dec;12(12):1-12  WB ;  Human.   PubMed:34887392 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.  IF&WB ;  Human.   PubMed:30791957 [IF=3.929] Chen Z et al. Generation of an iPSC line (SKLOi001-A) from a patient with CLCN2-related leukoencephalopathy. Stem Cell Res. 2020 Mar 17;45:101769.  ICC ;  Human.   PubMed:32278302 [IF=3.905] Xiong Xiao. et al. Construction of extracellular matrix-based 3D hydrogel and its effects on cardiomyocytes. Exp Cell Res. 2021 Nov;408:112843  IHC ;  Mouse.   PubMed:10.1016/j.yexcr.2021.112843 [IF=3.811] Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18.  WB ;  Human&Mouse.   PubMed:31853695 [IF=3.44] Li X et al. A comparative study of the behavior of neural progenitor cells in extrusion-based in vitro hydrogel models. Biomed Mater. 2019 Sep 9;14(6):065001.  WB ;  Mouse.   PubMed:31498778 [IF=3.225] Lin Li. et al. Breast cancer stem cells-derived extracellular vesicles affect PPARG expression by delivering microRNA-197 in breast cancer cells. Clin Breast Cancer. 2022 Feb;:  IF ;  Human.   PubMed:35279406 [IF=3.182] Liu K et al. Verteporfin suppresses the proliferation, epithelial-mesenchymal transition and stemness of head and neck squamous carcinoma cells via inhibiting YAP1. Journal of Cancer. 2019; 10(18): 4196-4207.  WB ;  Human.   PubMed:doi:10.7150/jca.34145 [IF=2.93] Tang, Xin, et al. "Downregulation of CXCR7 inhibits proliferative capacity and stem cell-like properties in breast cancer stem cells." Tumor Biology (2016): 1-9.  WB ;  Human.   PubMed:27460092 [IF=2.634] Ma C et al. Identification and Multilineage Potential Research of a Novel Type of Adipose-Derived Mesenchymal Stem Cells from Goose Inguinal Groove. DNA Cell Biol. 2018 Sep;37(9):731-741.  ICF ;  Goose.   PubMed:30102556 [IF=2.479] Wenjia Fang. et al. Prognostic value of OCT4 in colorectal cancer: analysis using immunohistochemistry and bioinformatics validation. 2020 Oct 14  IHC ;   PubMed:33185466 [IF=1.587] Ping Xu. et al. Generation and characterization of two iPSC lines carrying heterozygous or homozygous nonsense mutation in PROM1 gene from a single family. STEM CELL RES. 2022 Sep;:102913  WB ;  Human.   PubMed:36191543 [IF=1.461] Pei W et al. Biological characterization and pluripotent identification of ovine amniotic fluid stem cells. Cytotechnology. 2018 Jun;70(3):1009-1021.  IF&FCM ;  ovine embryo.   PubMed:29502286
Research Area	Cell biology  Chromatin and nuclear signals  Signal transduction  Stem cells  Cyclin  transcriptional regulatory factor  Cell type markers  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human OCT4: 201-300/360
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state. Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Subunit: Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Subcellular Location: Nucleus.Note=Expressed in a diffuse and slightly punctuate pattern. Tissue Specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Post-translational modifications: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation (By similarity). ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. Similarity: Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: Q01860 Gene ID: 5460 Database links: Entrez Gene: 282316 Cow Entrez Gene: 5460 Human Entrez Gene: 18999 Mouse Entrez Gene: 100127461 Pig Entrez Gene: 294562 Rat Omim: 164177 Human SwissProt: O97552 Cow SwissProt: Q01860 Human SwissProt: P20263 Mouse SwissProt: Q9TSV5 Pig Unigene: 249184 Human Unigene: 632482 Human Unigene: 646545 Human Unigene: 17031 Mouse Unigene: 161748 Rat
Product Picture	Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Cerebellum (Mouse) Lysate at 40 ug Lane 4: Testis (Mouse) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Heart (Rat) Lysate at 40 ug Lane 7: Cerebellum (Rat) Lysate at 40 ug Primary: Anti-Oct4 (SL0830R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 47 kD Blank control: Hela. Primary Antibody (green line): Rabbit Anti-4-Oct antibody (SL0830R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (Black line): Mouse spleen (Black). Primary Antibody (green line): Rabbit Anti-Oct4 antibody (SL0830R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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