Product Name	Lysozyme
Chinese Name	溶菌酶抗体
Alias	1 4 beta n acetylmuramidase c; 14 beta N acetylmuramidase; 14 beta N acetylmuramidase C; EC 3.2.1.17; lysosyme; Lysozyme C; Lysozyme C precursor; Lyz; LZM; Muramidase.
literatures	Specific References  (3)     |     SL0816R has been referenced in 3 publications. [IF=8.713] Peng Yang. et al. Single-cell RNA sequencing analysis of shrimp immune cells identifies macrophage-like phagocytes. ELIFE. 2022 Oct  WB ;  Shrimp.   PubMed:36200862 [IF=3.757] Haruna Nakayama. et al. Preparing a pore-size-controlled TEMPO oxidized cellulose nanofiber substrate for enzyme immunoassay applications. PROCESS BIOCHEM. Process Biochem. 2022 Jun;117:174  ELISA ;  Material.   PubMed:10.1016/j.procbio.2022.04.007 [IF=1.967] Nagata, Kenichiro. et al. Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie Brilliant Blue G-250 and R-250 dyes. ANAL SCI. 2022 Nov;:1-8  WB ;   PubMed:36451064
Research Area	Tumour  Cell biology  immunology  Kinases and Phosphatases  Synthesis and Degradation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Chicken, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Full length native Lysozyme: purufied protein
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Lysozyme catalyzes the hydrolysis of certain mucopolysaccharides of bacterial cell walls. Specifically, it catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N acetylmuramic acid and N acetylglucosamine. It is found in spleen, lung, kidney, white blood cells, plasma, saliva, milk, and tears. Subcellular Location: Secreted. DISEASE: Defects in LYZ are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Similarity: Belongs to the glycosyl hydrolase 22 family. SWISS: P00698 Gene ID: 396218 Database links: Entrez Gene: 4069 Human Entrez Gene: 17105 Mouse Entrez Gene: 17110 Mouse Omim: 153450 Human SwissProt: P61626 Human SwissProt: P08905 Mouse Unigene: 524579 Human 溶菌酶（lysozyme ）又称细胞壁质酶（muramidase）或n-乙酰胞壁质聚糖水解酶（n-acetylmuramideglycanohydralase）。 对于哺乳动物溶菌酶，其牛、猪、猫、兔、猴、马、羊等动物的乳汁中分离出溶菌酶，其化学性质与人溶菌酶相似。 溶菌酶作为机体非特异免疫因子之一，参与机体多种免疫反应，在机体正常防御功能和非特异免疫中，具有保持机体生理平衡的重要作用。可改善和增强巨嗜细胞吞噬和消化功能，激活白细胞吞噬功能，并能改善细胞抑制剂所导致的白细胞减少，从而增强机体的抵抗力。
Product Picture	Sample: Raw264.7 Cell (Mouse) Lysate at 40 ug HL-60 Cell (Human)Lysate at 40 ug Primary: Anti-Lysozyme (SL0816R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 16 kD Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Lysozyme Polyclonal Antibody, Unconjugated(SL0816R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Lysozyme Polyclonal Antibody, Unconjugated(SL0816R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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