Product Name	CD56
Chinese Name	神经Cell adhesion molecule1抗体
Alias	CD56 antigen; Cell Adhesion Molecule Neural 1; MSK39; N CAM 120; N CAM 140; N CAM 180; NCAM 1; NCAM 120; NCAM 140; NCAM1; Neural cell adhesion molecule 1 120 kDa isoform; Neural cell adhesion molecule 1 140 kDa isoform; Neural cell adhesion molecule 1; NCAM1_HUMAN.
literatures	Specific References  (3)     |     SL0736R has been referenced in 3 publications. [IF=5.68] Shi, Liang, et al. "Targeting Androgen Receptor (AR)→ IL12A Signal Enhances Efficacy of Sorafenib Plus NK Cells-Immunotherapy to Better Suppress HCC Progression." Molecular Cancer Therapeutics (2016): molcanther-0706.  IHC-P ;  Human.   PubMed:26939703 [IF=3.886] Huiqian Zeng. et al. Increased uterine NLRP3 inflammasome and leucocyte infiltration in a rat model of preeclampsia. 2021 Aug 10  IHC ;  Rat.   PubMed:34375018 [IF=3.26] Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).  IHC-P ;  Chicken.   PubMed:27215404
Research Area	Cell biology  Neurobiology  Stem cells  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	92kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD56/NCAM1: 701-800/858
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011] Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Subcellular Location: Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted. Similarity: Contains 2 fibronectin type-III domains. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P13591 Gene ID: 4684 Database links: Entrez Gene: 4684 Human Entrez Gene: 17967 Mouse Entrez Gene: 24586 Rat Omim: 116930 Human SwissProt: P13591 Human SwissProt: P13595 Mouse SwissProt: P13596 Rat Unigene: 503878 Human Unigene: 711235 Human Unigene: 733031 Human Unigene: 439182 Mouse Unigene: 4974 Mouse Unigene: 11283 Rat 细胞粘附蛋白（Call Adhesion Protein） 神经元Maker NCAM-1为神经Cell adhesion molecule，主要分布于神经组织，神经—肌肉接头，神经—内分泌腺和某些内分泌腺以及大多数神经外胚层来源的细胞、组织和Tumour中。 NCAM 1主要用于视网膜母细胞瘤、髓母细胞瘤、星形细胞瘤、神经母细胞瘤等Tumour方面的研究。CD56（神经细胞黏附分子，NCAM）表达于大部分神经外胚层来源的细胞系、组织和Tumour如视网膜母细胞瘤、成神经管细胞瘤、星形细胞瘤以及成神经细胞瘤。它也表达于一些中胚层来源的Tumour如横纹肌肉瘤。NK细胞以及NK细胞淋巴瘤为阳性。CD56被认为是NK细胞Tumour的重要Maker。该抗体识别分子量为180，145和125kDa亚型。
Product Picture	Protein: Hela(human)cell lysates at 40ug; Primary: rabbit Anti-CD56 (SL0736R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size:92 kD Observed band size:125 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NCAM1 Polyclonal Antibody, Unconjugated(SL0736R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control:Jurkat cells(blue). Primary Antibody:Rabbit Anti- CD56 antibody(SL0736R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (SL0736R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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