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Product Name CIDEB Chinese Name 细胞死亡激活剂CIDE-B抗体 Alias cell death activator CIDE-B; Cell death activator CIDE B; Cell death inducing DFFA like effector B; CIDEB_HUMAN; cell death-inducing DFFA-like effector B; Cide-b. literatures Specific References (1) | SL0716R has been referenced in 1 publications.[IF=2.742] Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7 WB ; Mouse.Research Area Tumour Cell biology Chromatin and nuclear signals Apoptosis Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 24kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CIDEB: 118-219/219 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as an inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE A and CIDE B were recently identified. CIDE contains a new type of domain termed CIDE N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE B induces apoptosis, which is inhibited by DFF45. CIDE B is a DFF45 inhibitable effector that promotes cell death and DNA fragmentation. CIDE B is expressed mainly in liver and small intestine and at lower levels in spleen, colon, kidney, peripheral blood lymphocytes, and bone marrow.
Function:
Activates apoptosis.
Subunit:
Inhibited by DFFB. Interacts with DFFA and DFFB.
Tissue Specificity:
Highly expressed in liver and small intestine and, at lower levels, in colon, kidney and spleen.
Similarity:
Contains 1 CIDE-N domain.
SWISS:
Q546V8
Gene ID:
27141
Database links:Entrez Gene: 27141 Human
Entrez Gene: 12684 Mouse
Omim: 604441 Human
SwissProt: Q546V8 Human
SwissProt: Q9UHD4 Human
SwissProt: O70303 Mouse
Unigene: 642693 Human
Unigene: 696081 Human
Unigene: 708040 Human
Unigene: 466766 Mouse
Unigene: 204016 Rat
CIDEB是在Apoptosis的过程中诱导分泌的一种蛋白,在棕色脂肪组织,肝脏和肾脏中发现有高水平的DFF45-like effector--DFF45样效应子b蛋白,简称cideb。CIDEB具有调控脂质代谢的作用。Product Picture Sample:
Large intestine (Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- CIDEB (SL0716R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 27 kD
Sample:
Lane 1: Rat Small intestine tissue lysates
Lane 2: Human HL-60 cell lysates
Primary: Anti-CIDEB (SL0716R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 27 kDa
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CIDEB Polyclonal Antibody, Unconjugated(SL0716R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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