Product Name	NF-M
Chinese Name	中分子量神经丝蛋白抗体
Alias	Neurofilament medium polypeptide; 160 kDa neurofilament protein; neurofilament 3; Nefm; 150kDa medium; NEF 3; NEF3; NEFM; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament protein medium; Neurofilament triplet M protein; Neurofilament3; NF M; NF160; Neurofilament-M; Neurofilament M; NFM; NFM_HUMAN.
literatures	Specific References  (4)     |     SL0710R has been referenced in 4 publications. [IF=3.659] Wang, Xiao-feng. et al. Active constituent of Polygala tenuifolia attenuates cognitive deficits by rescuing hippocampal neurogenesis in APP/PS1 transgenic mice. Bmc Complem Altern M. 2021 Dec;21(1):1-15  IF ;  Mouse.   PubMed:34696749 [IF=2.88] Gao, Yuhua, et al. "Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells." BioMed Research International 2013 (2013).  Chicken.   PubMed:23984389 [IF=1.06] Yuan, Quan, et al. "Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system." Bioscience, Biotechnology, and Biochemistry (2017): 1-8.  WB ;  Human.   PubMed:28394221 [IF=0] Kang J et al. Matrine protects retinal ganglion cells from apoptosis in experimental optic neuritis. 29 August 2019, PREPRINT (Version 1) available at Research Square.  IHF-P ;  Rat.   PubMed:doi:10.21203/rs.2.13687/v1
Research Area	Cell biology  immunology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test  IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	102kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NF-M: 101-200/916
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. Post-translational modifications: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Similarity: Belongs to the intermediate filament family. SWISS: P12839 Gene ID: 4741 Database links: Entrez Gene: 281347 Cow Entrez Gene: 4741 Human Entrez Gene: 18040 Mouse Entrez Gene: 24588 Rat Omim: 162250 Human SwissProt: O77788 Cow SwissProt: P07197 Human SwissProt: P08553 Mouse SwissProt: P12839 Rat Unigene: 458657 Human Unigene: 10971 Rat
Product Picture	Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-NF-M (SL0710R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 112 kD Paraformaldehyde-fixed, paraffin embedded (Cerebellum of rats); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-NF-M ) Polyclonal Antibody, Unconjugated (SL0710R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NFM) Polyclonal Antibody, Unconjugated (SL0710R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NF-M) Polyclonal Antibody, Unconjugated (SL0710R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat cochlea tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NF-M/Neurofilament M Polyclonal Antibody, Unconjugated(SL0710R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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