Product Name	LDLR
Chinese Name	低密度Lipoprotein受体抗体
Alias	LDLR_HUMAN; Low-density lipoprotein receptor; LDL receptor; FH; FHC; FHCL1; LDLCQ2; low density lipoprotein receptor;
literatures	Specific References  (11)     |     SL0705R has been referenced in 11 publications. [IF=6.78] Shuzhou Wang. et al. Effects of NAC assisted insulin on cholesterol metabolism disorders in canine type 1 diabetes mellitus. LIFE SCI. 2022 Dec;:121193  WB ;  Dog.   PubMed:36463942 [IF=5.34] Wei Zou. et al. Imperatae rhizoma-Hedyotis diffusa Willd. herbal pair alleviates nephrotic syndrome by integrating anti-inflammatory and hypolipidaemic effects. Phytomedicine. 2021 Sep;90:153644  WB ;  Rat.   PubMed:34274601 [IF=4.268] Grube,et al.Mining the Secretome of C2C12 Muscle Cells: Data Dependent Experimental Approach To Analyze Protein Secretion Using Label-Free Quantification and Peptide Based Analysis.(2018) Journal of Proteome Research. 17:879-890.  WB ;  Mouse.   PubMed:29322779 [IF=4.081] Huo Yihui. et al. Effect of Copper Exposure on the Cholesterol Metabolism in Broiler Liver. BIOL TRACE ELEM RES. 2023 Mar;:1-9  WB ;  Chicken.   PubMed:36929115 [IF=2.97] Wu, Hao, et al. "The antihypercholesterolemic effect of jatrorrhizine isolated from Rhizoma Coptidis." Phytomedicine (2014).  WB ;   PubMed:24894270 [IF=2.74] Kyoung Jin Leeet al. Real-time monitoring of oncolytic VSV properties in a novel in vitro microphysiological system containing 3D multicellular tumor spheroids. PLoS One . 2020 Jul 6;15(7):e0235356.  IF ;  Human.   PubMed:32628693 [IF=2.7] Kou, Shuming, et al. "Synergetic cholesterol-lowering effects of main alkaloids from Rhizoma Coptidis in HepG2 cells and hypercholesterolemia hamsters." Life Sciences (2016).  WB ;  Human.   PubMed:26876917 [IF=2.65] Huiming Hu. et al. The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats.. EVID-BASED COMPL ALT. 2022 Nov;2022:3033311-3033311  WB ;  Rat.   PubMed:36425260 [IF=2.629] Mei Hui. et al. The Hypolipidemic Effect of Dalbergia odorifera T. C. Chen Leaf Extract on Hyperlipidemic Rats and Its Mechanism Investigation Based on Network Pharmacology. Evid-Based Compl Alt. 2021;2021:3155266  WB ;  Rat.   PubMed:34987591 [IF=1.55] Jia, Lianqun., et al. "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016).  IHC-P ;  Rat.   PubMed:27082100 [IF=] Kwon,et al.Luterion and separating and culturing methods for same.(2017) . :.  IF ;  Plant.   PubMed:US20170361243A1
Research Area	Tumour  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test ICC=1:100  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	92kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LDL-R: 781-860/860 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2022] Function: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Subunit: Interacts with LDLRAP1. Interacts with SNX17. Interacts with HCV E1/E2 heterodimer. Interacts with HIV-1 Tat. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein. Note=Found distributed from the plasma membrane to intracellular compartments. Tissue Specificity: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Post-translational modifications: N- and O-glycosylated. Ubiquitinated by MYLIP leading to degradation. Similarity: Belongs to the LDLR family. Contains 3 EGF-like domains. Contains 7 LDL-receptor class A domains. Contains 6 LDL-receptor class B repeats. SWISS: P01130 Gene ID: 3949 Database links: Entrez Gene: 3949 Human Entrez Gene: 16835 Mouse Entrez Gene: 300438 Rat Omim: 606945 Human SwissProt: P01130 Human SwissProt: P35951 Mouse SwissProt: P35952 Rat Unigene: 213289 Human Unigene: 728190 Human Unigene: 3213 Mouse Unigene: 10483 Rat 低密度Lipoprotein受体 LDLR是一种存在于细胞表面的、可识别多种配体的Lipoprotein受体，在体内对于富含甘油三酯的Lipoprotein代谢非常重要; LDL R目前主要用于代谢及Tumour方面的研究。
Product Picture	Sample: K562(Human) Cell Lysate at 30 ug Raw264.7(Mouse) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug SW480(Human) Cell Lysate at 30 ug Primary: Anti-LDL receptor (SL0705R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kD Observed band size: 120 kD Sample:Human colon Lysate at 45ug; Primary: Anti-LDL receptor (SL0705R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 92kD Observed band size : 92kD HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (LDL receptor) polyclonal Antibody, Unconjugated (SL0705R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control：A431. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL0705R),Dilution: 1μg /10^6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody:Goat anti-rabbit IgG-AF647,Dilution: 1μg /test. Protocol A431 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (black line): HepG2 (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary Antibody (green line): Rabbit Anti-LDLreceptor antibody (SL0705R) ; Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1μg /test. Blank control: Raw264.7. Primary Antibody (green line): Rabbit Anti-LDL receptor antibody (SL0705R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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