Rabbit Anti-NSBP1 antibody
Nucleosome-binding protein 1; Nucleosome-binding protein 45; NBP-45; GARP45 protein; HMGN5_HUMAN.
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Product Name NSBP1 Chinese Name 核小体Binding protein1抗体 Alias Nucleosome-binding protein 1; Nucleosome-binding protein 45; NBP-45; GARP45 protein; HMGN5_HUMAN. Research Area immunology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NSBP1: 201-282/282 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NSBP1 binds specifically to nuclear core particles and acts as a transcriptional activator. [Subcellular Location] Nucleus. [Tissue Specificity] Widely expressed with highest levels in submaxillary gland, thymus, kidney and liver and lowest levels in brain, lung, pancreas and eye. [Developmental Stage] Highest levels are found in 7-day-old embryos. Levels in the 7-day-old embryo are 4-fold higher than in the adult and almost 10-fold higher than in later embryonic stages. [SIMILARITY] Belongs to the HMGN family.
Function:
Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction (By similarity).
Subcellular Location:
Nucleus. Note=Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin (By similarity).
Tissue Specificity:
Ubiquitously expressed.
Similarity:
Belongs to the HMGN family.
SWISS:
P82970
Gene ID:
79366
Database links:Entrez Gene: 79366 Human
Omim: 300385 Human
SwissProt: P82970 Human
Unigene: 282204 Human
NSBP-1主要用于前列腺疾病方面的研究。Product Picture 
Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSBP1 ) Polyclonal Antibody, Unconjugated (SL0700R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NSBP1 Polyclonal Antibody, Unconjugated(SL0700R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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